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High resolution chromosomes from first trimester trophoblast cultures |
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| Authors: | Finn Søndergaard Merete Kristensen Niels Tommerup |
| Institution: | The John F. Kennedy Institute, DK 2600 Glostrup, Denmark |
| Abstract: | A simple method for obtaining prometaphase chromosomes from cultured first trimester cells involves the addition of BrdU and FdU 11 h before harvest and ethidium bromide 1.5 h before harvest. High resolution R-banding is obtained by acridine orange staining. |
| Keywords: | Prenatal diagnosis First trimester Trophoblast tissue High resolution chromosomal analysis BrdU Ethidium bromide |