| 双酚A体外类雌激素活性评价及其作用机制的初步研究 |
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| 引用本文： | 朱毅,舒为群,卓鉴波,余楠,刘波.双酚A体外类雌激素活性评价及其作用机制的初步研究[J].重庆环境科学,2003,25(4):20-22. |
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| 作者姓名： | 朱毅 舒为群 卓鉴波 余楠 刘波 |
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| 作者单位： | [1]重庆市第三军医大学预防医学系环境卫生学教研室 [2]重庆市三峡库区生态环境与人群健康研究重点实验室，重庆400038 |
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| 基金项目： | 国家自然科学基金 (3980 0 1 2 7) |
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| 摘 要： | 采用体外MCF-7细胞增殖试验检测了双酚A(BPA)的类雌激素活性，并用生长曲线分析、细胞周期分析、他奠昔芬拮抗试验和凋亡检测对其作用机制进行了初步探讨。结果显示BPA有明显刺激MCF-7细胞增殖的类雌激素活性；细胞周期分析BPA组细胞增殖指数均明显高于溶剂对照组；BPA刺激MCF-7细胞增殖的类雌激素活性可被Tam拮抗：BPA能明显抑制MCF-7细胞凋亡的发生。结果提示BPA刺激MCF-7细胞增殖的类雌激素活性作用机制可能是BPA与雌激素受体结合后，影响细胞增殖及细胞凋亡过程，从而产生一系列生物效应。
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| 关 键 词： | 环境雌激素 双酚A MCF－7细胞增殖试验 他莫昔芬 细胞凋亡 类雌激素 活性评价 |
| 文章编号： | 1001-2141(2003)04-0020-03 |
| 修稿时间： | 2002年9月28日 |
Evaluation of the Estrogenic Activity of Bisphenol A in Vitro and Study on the Mechanisms |
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| Zhu Yi,Shu Weiqun,Zhuo Jianbo,Yu Lan,Liu Bo.Evaluation of the Estrogenic Activity of Bisphenol A in Vitro and Study on the Mechanisms[J].Chongqing Environmental Science,2003,25(4):20-22. |
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| Authors: | Zhu Yi Shu Weiqun Zhuo Jianbo Yu Lan Liu Bo |
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| Abstract: | The estrogenic activity of bisphenol A (BPA) was determined by cell proliferation test of MCF 7 cells in vitro.To study the mechanisms by analysis of growth curve,cell cycle,tamoxifen (Tam) resistant test and apoptosis detection method.The results were as following:the estrogenic activity of BPA was observed.The cell proliferation index of BPA was higher than alcohol in analysis of cell cycle.Tam could resist the estrogenic activity of BPA.BPA could inhibit the cell spoptosis of MCF 7 cells.The results suggested that the estrogenic activity of BPA may be due to binding to the estrogen receptor,which may influence cell proliferation and apoptosis. |
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| Keywords: | environmental estrogen(EE) bisphenol A(BPA) cell proliferation test of MCF 7 cells Tamoxifen(Tam) apoptosis |
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