高效液相色谱法检测土壤中的壬基酚

建立一种土壤中壬基酚的提取和含量测定方法。土壤样品经二氯甲烷索氏提取、旋转蒸发仪浓缩蒸干后，用色谱流动相溶解。以色谱甲醇与高纯水（体积比为85：15）为流动相，经C18色谱柱分离，在检测波长277nm进行紫外光检测。土壤样品中4-壬基酚的捡出限为20ng／g，4-壬基酚混合标准检测相对标准偏差范围在2．39％-4．56％之间。加入标准溶液后，土壤中壬基酚的加标回收率为98．73％～101．78％，标准偏差小于2．97％。该方法简便快速，灵敏可靠，适用于土壤样品中环境类激素4-壬基酚的含量检测。

Determination of 4-Nonylphenol in Soil by High Performance Liquid Chromatography

A simple and highly sensitive method has been developed for the determination of 4 -nonylphenol （4 -NP）in soil by high performance liquid chromatography（HPLC） detection. Soil samples extracted by the method named Soxhlet with dichloromethane, and evaporated by rotary evaporators. Finally, the chromatographic mobile phase was used to dissolve the sample. The HPLC detection use methanol and water（volume ratio 85：15）as mobile phase. Samples separated by the C18 column, and detection in the wavelength of 277 nm for UV detection. The detection hmit of 4 - Nonylphenol in Soil samples is 20 ng/g. The relative standard deviation range of detection of 4 - nonylphenol mixed standard is between 2. 39% -4. 56%. Adding standard solution, the recovery rate of nonylphenol in soil was 98. 73% - 101.78%, the standard deviation less than 2. 97%. These results demonstrated that the proposed method is simple, sensitive, and reliable for the determination of 4 -NP in soil.