Identification of Four Distinct Subunit Types in the Unique 6×6 Hemocyanin of the Centipede Scutigera coleoptrata |
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Authors: | W Gebauer J Markl |
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Institution: | (1) Institute of Zoology, University of Mainz, D-55099 Mainz, Germany, DE |
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Abstract: | We isolated 6×6 hemocyanin, dissociated it into subunits, and examined it by electron microscopy. The subunits were separated
by native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate PAGE, and crossed immunoelectrophoresis. Single
subunits were isolated by gel cutting from native PAGE and identified as hemocyanin by measuring their ultraviolet spectrum.
A total of four distinct hemocyanin subunits were identified, and the subunit pattern of the three electrophoresis systems
assigned to each other. The relative proportion of subunits a:b:c:d were 2 : 2 :>: 1 as determined by densitometry. Presumably, c and d act as linkers between hexamers.
Received: 9 November 1998 / Accepted in revised form: 14 April 1999 |
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