Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis |
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Authors: | Yansong Hou Wei Liang Liping Zhang Shuiping Cheng Feng He and Zhenbin Wu |
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Institution: | (1) Institute of Oceanology, Polish Academy of Sciences, Powstaców Warszawy 55, 81-712 Sopot, Poland |
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Abstract: | The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem
and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on
a reversephase C18 nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes,
and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide a, 19′-butanoyloxyfucoxanthin, chlorophyll c
1 + c
2, phephorbides a, peridinin, methyl-chlorophyllide a, fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b allomer, chlorophyll b, chlorophyll a allomer, chlorophyll a, and β,β-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis
could be a useful tool for monitoring phytoplankton communities and their abundance. |
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Keywords: | |
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