| 基于藻类荧光的毒性测试研究进展 |
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| 引用本文： | 史媛,王英才,李晔,陈水松,叶丹.基于藻类荧光的毒性测试研究进展[J].环境科学与技术,2013(3):59-64. |
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| 作者姓名： | 史媛 王英才 李晔 陈水松 叶丹 |
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| 作者单位： | 武汉理工大学资源与环境工程学院;长江流域水资源保护局长江流域水环境监测中心 |
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| 基金项目： | 国家科技支撑项目:南水北调中线工程水源地及沿线水质监测预警关键技术研究与示范(2011BAC12B00);水利部公益性行业专项经费项目:长江中下游干流纳污总量控制研究(201001006) |
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| 摘 要： | 毒性测试以微藻细胞为生物敏感元件,以叶绿素荧光变化为响应指标,在监测水体重金属和农药污染方面,具有检出限低、重复性好等优点。然而,实际水环境中藻类生长受众多因素的影响,此法仅能反应各种污染因子的综合毒性。近几年,众多学者致力于提高微藻生物监测的特异性以确定污染物种类,改善微藻固定技术以适应原位监测。文章介绍了基于微藻荧光的毒性测试研究进展,展望了未来的发展趋势。。
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| 关 键 词： | 毒性测试 叶绿素荧光 环境监测 细胞固定 特异性 |
Progress on Detecting Toxicity by Algae Fluorescence |
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| SHI Yuan,WANG Ying-cai,LI Ye,CHEN Shui-song,YE Dan.Progress on Detecting Toxicity by Algae Fluorescence[J].Environmental Science and Technology,2013(3):59-64. |
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| Authors: | SHI Yuan WANG Ying-cai LI Ye CHEN Shui-song YE Dan |
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| Institution: | 1.College of Resource and Environmental Engineering,Wuhan University of Technology,Wuhan430070,China;2.Water Environment Monitoring Centre of Yangtze River Basin,Yangtze River Valley Water Resource Protection Bureau,Wuhan430010,China) |
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| Abstract: | Inhibition test,quantifying the toxicity of pollutants by variation of chlorophyll fluorescence,shows a low detection limit and good reversibility in detecting heavy metals and herbicides.However it exhibits a summary response over a range of toxic substances.In recent years,many researchers have focused on improving the specificity to specify the kinds of pollutants,and upgrading immobilization method allowing on-site field monitoring.Advances in the application of fluorescence-based biosensors in monitoring were reviewed,and its future trends were discussed. |
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| Keywords: | inhibition test chlorophyll fluorescence environmental monitoring cell immobilization specificity |
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