产表面活性剂根际菌协同龙葵修复镉污染土壤

从某矿区重金属污染的土壤中分离筛选出一株能产生物表面活性剂的根际细菌LKS06,经生理生化特征及16S rDNA序列分析,将其鉴定为假单孢菌属(Pseudomonas sp.LKS06).生物学特性的测定表明,菌株LKS06对多种重金属有很高的耐受性,且具有分泌吲哚乙酸、产铁载体和ACC脱氨酶活性的能力,并对土壤重金属...

Biosurfactant-producing Rhizobacteria Assisted Solanum nigrum L.Phytoremediation of Cd-polluted Soils

A biosurfactant-producing rhizobacteria LKS06 was isolated and selected from heavy metal contaminated soils of a mine and identified as Pseudomonas sp.based on analysis of its physical and biochemical characteristics and its 16S rDNA gene sequence.The strain LKS06 which was found to exhibit high tolerance of different heavy metals had the capacity to produce indole acetic acid(IAA),siderophore and 1-aminocyclopropane-1-carboxylate(ACC)deaminase,and also could activate heavy metals effectively when added to contaminated soils.Subsequently,a pot experiment was done for investigating the influence of inoculating of the strain LKS06 on plant growth and Cd uptake by hyperaccumulator Solanum nigrum L.in soils artificially contaminated with different levels of Cd(0,10,25,50,100 mg/kg).Results showed that inoculation of strain LKS06 stimulated the growth of plants,and Cd uptake by plants significantly increased as well.Compared to non-inoculated control,dry weights of root,tem and leaves tissues of plant inoculated with strain LKS06 were increased from 6.3% to 11.9%,10.0% to 18.8% and from 4.8% to 23.7% respectively.Moreover,the largest total Cd contents of the root and above-ground tissues were increased by 36.7% and 42.4% respectively.The study using plant-microbe partnerships for elevating the efficiency of phytoremediation has provided a new approach for remediation of heavy metal contaminated soils.