| 基因工程菌在SMBR中对直接耐晒蓝的脱色 |
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| 引用本文： | 丁华,金若菲,周集体.基因工程菌在SMBR中对直接耐晒蓝的脱色[J].环境科学与技术,2007,30(6):66-68. |
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| 作者姓名： | 丁华 金若菲 周集体 |
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| 作者单位： | 大连理工大学环境与生命学院,大连,116024 |
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| 摘 要： | 文章优化了基因工程菌Escherichia coli JM109(pGEX-AZR)对三偶氮染料直接耐晒蓝(B2RL)的脱色条件,并考察其在SMBR中对直接耐晒蓝的脱色效果。研究表明,最佳脱色条件为:进水浓度400mg/L,初始生物量5g/L,pH7.0～8.0,葡萄糖浓度0.5g/L。在此条件下,基因工程菌在SMBR中对直接耐晒蓝的脱色率稳定在90%以上,对COD的去除率范围50%～62%。系统运行过程中,反应器内生物量稳定在3.90g/L左右;酶活力和EPS均呈现先增大,再降低,最后较为平缓的趋势;系统运行周期约为25d。
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| 关 键 词： | 基因工程菌 偶氮染料 SMBR脱色 |
| 文章编号： | 1003-6504（2007）06-0066-03 |
| 修稿时间： | 2006-09-052006-10-10 |
Decolorization of B2RL Using SMBR with a Gene-engineered Strain |
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| DING Hua,JIN Ruo-fei,ZHOU Ji-ti.Decolorization of B2RL Using SMBR with a Gene-engineered Strain[J].Environmental Science and Technology,2007,30(6):66-68. |
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| Authors: | DING Hua JIN Ruo-fei ZHOU Ji-ti |
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| Abstract: | This paper relates to a gene-engineered bacteria strain - Escherichia coli JM109 (pGEX-AZR), and its application to degradation of pharmaceutical wastewater with respect to color of an azo dye. The experiment shows the very efficient in both color and COD removals of the strain which plays a vital part in strengthening EPS and enzyme activity of biomass in the sequence MBR. |
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| Keywords: | gene-engineered bacteria strain azo dye SMBR decolorization |
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