高效硫酸盐还原菌的分离及特性研究

采用叠皿夹层法,从糖蜜酒精废水厌氧反应器的污泥中分离出一株硫酸盐还原菌,命名为SRB-22。该菌株的菌体形态呈杆状和弧状,革兰氏染色阴性,属于兼性厌氧菌,初步确定为脱硫弧菌属(Desulfovibrio)。在不含Fe2+的培养基中,菌株4h后进入对数生长期,32h达到生长最高峰。探讨了SO42-初始浓度、COD/SO42-比值、Fe2+初始浓度对SRB-22硫酸盐去除率的影响,结果表明:在一定浓度范围内,SO42-初始浓度越大,菌株完全去除SO42-所需时间也越长,当SO42-浓度大于6g/L时,菌株对SO42-的去除率明显降低;随着COD/SO42-比值的增加,SO42-的去除率呈上升的趋势,COD/SO42-值达到2.4时,培养5d后即可较好地还原SO42-;在一定浓度范围内,随Fe2+初始浓度的增大,SO42-的去除率增加。当Fe2+浓度达到1.0g/L以上,SO42-的去除率迅速下降。

Isolation of High Efficient Sulfate-reducing Bacteria and Its Biological Desulfurization Capability

A strain of sulfate reducing bacteria named SRB-22 was isolated from granular sludge of anaerobic reactor of molasses wastewater using the method of repeat dish sandwich cultivated. It was identified that SRB-22 is rod or arc single cell,gram-negative,annex anaerobes of Desulfovibrio sp. The strain could reach the logarithmic phase after inoculation for 4h,and to a peak after 32h on the medium without Fe2+. Effect of SO42concentration,COD/SO42ratio and Fe2+ concentration on removal rate of SO42by the strain was studied. Results showed that the sulfate removal rate was raised with increase of SO42concentration,COD/SO42rate or Fe2+ concentration. When SO42concentration was more than 6g/L,the removal rate of SO42deceased obviously. When COD/SO42ratio was about 2.4 after cultivation for 5h,SO42could be reduced perfectly. When Fe2+ concentration was more than 1.0g/L,removal rate of SO42decreased sharply.