盐度对细菌菌株降解苯酚的影响

从处理苯酚废水活性污泥中筛选分离到四株苯酚降解优势细菌菌株JHCFS1,JHCFS2,JHCFS3与JHCFS4,通过四株细菌在不同盐度条件下的苯酚降解率表明,随着盐浓度的逐渐升高,抑制作用逐渐增大,当NaCl,KCl浓度为4%时,四株细菌降解苯酚均受到显著抑制。苯酚浓度为1000mg/L,NaCl浓度为3%时,JHCFS2的降解率最高为83%,JHCFS4的降解率最低为47.20%;KCl浓度为3%时,JHCFS2的降解率最高为99%,JHCFS4的降解率最低为48%,表明四株菌在盐浓度(NaCl,KCl)低于3%的条件下可正常降解苯酚。通过对四株菌的16S rRNA基因克隆与序列分析,在NCBI进行BLAST获得同源性序列,利用Clustalx1.8软件和MEGA4.0软件进行同源性比较和系统发育学分析,结合生理生化特性将菌株JHCFS1,JHCFS2,JHCFS3与JHCFS4(GenBank收录号:FJ455076,FJ455077,FJ458437与FJ458438)分别归为Bacillus simplex,Bacillus cereus,Bacillus pumilus与Bacillus cibi,为降解苯酚提供了微生物物种资源。

Influence on Phenol Degradation by Bacterial Strains under Different Salinity Conditions

Four phenol degradation bacterial strains named JHCFS1,JHCFS2,JHCFS3 and JHCFS4 were isolated from activated sludge in phenol wastewater treatment system.Phenol degradation efficiency of 4 bacterial strains were studied under different salinity conditions,results indicated that degradation efficiencies of the 4 bacterial phenol decreased with increasing salinity and degradation efficiency was the lowest when NaCl and KCl concentration was 4% respectively.When the phenol concentration was 1,000mg/L,NaCl concentration was 3%,strain JHCFS2 had the highest degradation efficiency which was 83% and JHCFS4 had the lowest degradation efficiency which was 47.20%.When KCl concentration was 3%,JHCFS2 had the highest degradation efficiency which was 99% and JHCFS4 had the lowest degradation efficiency which was 48%.The 16S rRNA gene of 4 bacterial strains was cloned and sequenced,phylogenetic tree was constructed using Clustalx1.8 software and MEGA 4.0 software,the bacterial strain combined with bacterial shape and physiological and chemical characteristics were classified to Bacillus simplex,Bacillus cereus,Bacillus pumilus and Bacillus cibi.