光合细菌黑暗好氧降解2-氯酚的特性研究

研究了光合细菌PSB-1D在黑暗好氧条件下处理2-氯苯酚时的降解规律和影响因素。考察了不同培养时间、碳源、氮源、初始pH值、PSB-1D接种量、摇床转速、不同初始2-氯苯酚浓度等因素对PSB-1D生长和2-氯苯酚处理效果的影响。结果表明:以葡萄糖、硫酸铵和酵母膏作为最佳碳氮营养源,初始pH值为7.0、接种量为10%、摇床转速为130r/min为最佳工艺条件,在此条件下PSB-1D处理2-氯苯酚降解率可达61.50%。黑暗好氧条件下光合细菌PSB-1D降解2-氯苯酚的规律符合一级降解动力学模型。随着初始2-氯苯酚的浓度逐渐由20mg/L提高到150mg/L,半衰期由39.36h延长为92.88h,也即2-氯苯酚浓度越高,降解所需的时间越长,降解速率常数Kb亦逐渐减小,由0.01754mg(/L·h)降到0.007458mg(/L·h)。

Factors of O-chlorophenol Biodegradation by Photosynthetic Bacteria under Dark and Aerobic Conditions

Kinetics of o-chlorophenol degradation and effects of related factors by photosynthetic bacteria PSB-1D under dark and aerobic conditions were studied. Factors including concentration of o-chlorophenol,carbon source,nitrogen source,initial pH,inoculation quantity,shaken table rotation rate were optimized for photosynthetic bacteria. Results showed that with glucose,ammonium sulfate and beef exact as optimal carbon source and nitrogen source,under the conditions of initial pH 7.0,inoculation quantity 10% and shaken table rotation rate 130r/min,the degradation rate can be up to 61.50%. The degradation process fitted the first order reaction equation. The greater the concentration of initial o-chlorophenol,the smaller the degradation rate constant,the longer the degradation half life is. When initial concentration of o-chlorophenol increased from 20mg/L to 150mg/L,the degradation half life was prolonged from 39.36h to 92.88h.