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| 2-酮基-L-古龙酸的高效液相色谱测定方法和条件 | |
| 引用本文： | 肖入峰,刘景洋,张建强.2-酮基-L-古龙酸的高效液相色谱测定方法和条件[J].环境科学与技术,2012,35(5):47-51. |
| 作者姓名： | 肖入峰 刘景洋 张建强 |
| 作者单位： | 1. 西南交通大学地球科学与环境工程学院,四川成都610031;中国环境科学研究院,北京100012 2. 中国环境科学研究院,北京,100012 3. 西南交通大学地球科学与环境工程学院,四川成都,610031 |
| 基金项目： | 国家水体污染控制与治理科技重大水专项，国际科技合作项目 |
| 摘 要： | 为了快速准确的检测维生素C(VC)生产工艺中VC的重要前体物2-酮基-L-古龙酸(2-KLG)浓度,采用高效液相色谱法进行测定,选用6×250 mm Carbohydrate分析柱,乙腈:磷酸二氢钾=60∶40的流动相,在波长210 nm处紫外光检测,控制流速为1 mL/min和温度45℃。结果表明2-KLG在6 min左右出峰,与VC具有较好分离度;2-KLG标准曲线的回归方程为y=0.776x-13.46,相关系数达到99.9%,检出限为0.5 mg/L;回收率为96.3%~104%,RSD为6.28%(n=5) |
| 关 键 词： | 高效液相色谱(HPLC)法 2-酮基-L-古龙酸(2-KLG) 维生素C(VC)前体物 |
Determination of 2-Keto-L-G-Gulconic Acid by HPLC |
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| XIAO Ru-feng , LIU Jing-yang , ZHANG Jian-qiang.Determination of 2-Keto-L-G-Gulconic Acid by HPLC[J].Environmental Science and Technology,2012,35(5):47-51. | |
| Authors: | XIAO Ru-feng LIU Jing-yang ZHANG Jian-qiang |
| Institution: | 1(1.Faculty of Geosciences and Environmental Engineering,Southwest Jiaotong University,Chengdu 610031,China; 2.Chinese Research Academy of Environmental Sciences,Beijing 100012,China) |
| Abstract: | A fast,accurate determination of 2-Keto-L-G-gulconic acid(2-KLG),the precursor of VC in fermentation process,is crucial to the VC production.A sophisticated tool of high performance liquid chromatography(HPLC) was thus used for the detection with a carbohydrate analysis column(6×250 mm) and the mobile phase(acetonitrile: potassium dihydrogen phosphate=60∶40) under the condition of 1 mL/min of flow rate at 45℃ and 210 nm of UV wavelength.As a result,chromatographic peak of 2-KLG came out at around six minutes along with a perfect range of recovery 96.3%~104% as well as RSD of 6.28%(n=5). |
| Keywords: | HPLC 2-Keto-L-G-gulconic acid(2-KLG) precursor of VC |
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