孔雀石绿诱发的蚕豆根尖细胞微核及染色体畸变

采用蚕豆根尖细胞微核试验和染色体畸变试验方法,研究孔雀石绿对蚕豆根尖细胞的致畸效应。实验结果表明:孔雀石绿浓度在0.05~2.0 mg/L之间时,蚕豆根尖细胞的微核率和染色体畸变率随着浓度的增加而升高。当达到1.0 mg/L时,微核率和染色体畸变率达到最高水平,随后,均开始下降。在同一浓度下,随着处理时间的不断延长,微核率和染色体畸变率不断增大。微核率在36 h时,达到最高水平,而染色体畸变率在48 h时达到峰值,之后下降。但仍显著高于对照组。

Micronucleus and Chromosome Aberration in Root-tip Cell of Vicia faba Induced by Malachite Green

Teratogenic effect of malchite green(MG) on Vicia faba root-tip cell was studied through MNT and chromosome aberration testing.Influences of concentration of MG on micro-nuclear rate(MCN) and chromosome aberration frequency(CAF) were examined.When MG concentration was increased to as high as 10 mg/L,both of the rates reached the top and then declined as MG concentration continued to rise.The time of Vicia faba root-tip exposed to malchite green was also investigated,i.e.,MCN attained the highest level after 36h exposure and so did CAF after 48 h,and thereafter both dropped,but still higher than the control groups.