| 秸秆沼气干发酵基质中微生物DNA提取方法筛选 |
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| 引用本文： | 申瑞霞,朱继英.秸秆沼气干发酵基质中微生物DNA提取方法筛选[J].环境科学与技术,2013(1):40-43. |
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| 作者姓名： | 申瑞霞 朱继英 |
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| 作者单位： | 山东理工大学农业工程与食品科学学院 |
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| 基金项目： | 国家自然科学基金(51008185);山东省中青年科学家科研奖励基金(BS2011NJ015);山东理工大学青年支持计划(110011) |
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| 摘 要： | 比较了4种处理方法对从秸秆沼气干发酵基质中提取微生物总DNA纯度和产量的影响,以及添加PVP和使用试剂盒对DNA粗提液的纯化效果,试图寻找一种适用于从秸秆沼气干发酵基质中提取微生物总DNA的有效方法。结果表明:采用冻融处理和酶处理相结合的方法得到DNA提取液的A260/A230和A260/A280相对较高,与其它3种方法有显著性差异(P≤0.05);提取液中添加PVP对去除DNA粗提液中的腐殖酸有一定效果,但添加量1%6%的效果差异不显著;经细菌DNA纯化试剂盒纯化后A260/A230和A260/A280分别达到(1.223±0.156)和(1.737±0.120)。对吸附了磷酸盐的氢氧化钽进行解吸,当pH=12时解吸率为52.45%。研究结果表明,氢氧化钽能够有效地去除水溶液中磷酸盐的吸附剂。
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| 关 键 词： | 秸秆 干发酵 微生物 DNA |
Extraction of Microbial DNA from Dry Anaerobic Digestion Substrate with Crop Straw |
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| SHEN Rui-xia,ZHU Ji-ying.Extraction of Microbial DNA from Dry Anaerobic Digestion Substrate with Crop Straw[J].Environmental Science and Technology,2013(1):40-43. |
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| Authors: | SHEN Rui-xia ZHU Ji-ying |
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| Institution: | (School of Agricultural and Food Engineering,Shandong University of Technology,Zibo 255049,China) |
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| Abstract: | To find an effective extracting method for microbial DNA from dry anaerobic digestion substrate with crop straw,purities and yields of microbial DNA extracted by 4 different treatments were compared.Results showed that A260/A230 and A260/A280 of the method combing with freeze-thaw processing and enzyme treatment were significantly higher than that of the other 3 methods(P≤0.05).Adding PVP to extraction buffer can slightly reduce the content of humic acid in DNA crude extracts,but effects of PVP addition from 1% to 6% have no significant difference.Value of A260/A230 and A260/A280 of DNA extraction purified by bacterial DNA purification kit reached to(1.223±0.156) and(1.737±0.120) respectively. |
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| Keywords: | crop straw dry anaerobic digestion microorganism DNA |
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