| 活性污泥中PHA合成优势菌的筛选及产物结构分析 |
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| 引用本文： | 李晓雁,黄海东,卢显芝,杨红澎,王燕森,曹晓娟.活性污泥中PHA合成优势菌的筛选及产物结构分析[J].环境科学与技术,2009,32(9). |
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| 作者姓名： | 李晓雁 黄海东 卢显芝 杨红澎 王燕森 曹晓娟 |
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| 作者单位： | 1. 天津农学院,天津,300384
2. 南开大学生命科学学院,天津,300071 |
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| 基金项目： | 天津市高等学校科技发展基金 |
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| 摘 要： | 聚羟基脂肪酸酯(PHA)是一种具有广阔应用前景的生物可降解塑料,活性污泥中存在大量PHA合成菌,通过好氧/微好氧/沉淀的方式对污泥进行驯化,使PHA占污泥MLVSS的比例提高到21.19%。用BOX-PCR、ERIC-PCR和REP-PCR指纹图谱技术,对驯化前后污泥样品的菌群组成进行比较分析,结合尼罗蓝荧光平板法和菌落原位杂交技术,筛选得到活性污泥中PHA合成的优势菌株CAMP11。分类鉴定表明CAMP11属于芽孢杆菌属,FT-IR与NMR碳谱分析表明其合成的产物为羟基丁酸与羟基戊酸的共聚物(PHBV)。将菌株CAMP11回注入污泥驯化液,可使PHA占污泥MLVSS的比例进一步提高到32.08%。
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| 关 键 词： | 聚羟基脂肪酸酯 污泥驯化 DNA指纹图谱 优势菌 |
Screening of Dominant Bacteria for PHA Accumulation in Activated Sludge and Structure Analysis of Its Product |
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| LI Xiao-yan,HUANG Hai-dong,LU Xian-zhi,YANG Hong-peng,WANG Yan-sen,CAO Xiao-juan.Screening of Dominant Bacteria for PHA Accumulation in Activated Sludge and Structure Analysis of Its Product[J].Environmental Science and Technology,2009,32(9). |
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| Authors: | LI Xiao-yan HUANG Hai-dong LU Xian-zhi YANG Hong-peng WANG Yan-sen CAO Xiao-juan |
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| Institution: | 1.Tianjin Agricultural University;Tianjin 300384;China;2.College of Life Sciences;Nankai University;Tianjin 300071;China |
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| Abstract: | Polyhydroxyalkanoates(PHA)are biodegradable thermoplastics with attractive application prospects.Many bacteria in activated sludge have the capacity to synthesize PHA,but the proportion of PHA-synthesis-bacteria with total microbe is very small.The numbers of PHA-synthesis-bacteria increased under the domestication of aerobic-anoxicsedimentary condition,with the percents of PHA/MLVSS increasing to 21.19%.Differences of microbial communities between native sludge and domesticated sludge were analyzed with th... |
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| Keywords: | polyhydroxyalkanoates(PHA) sludge domestication DNA fingerprint dominant bacteria |
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