白腐真菌降解染料反应器微生物群落结构研究

利用DGGE分子标记技术分析了两种白腐真菌生物反应器处理染料废水过程中生物膜微生物群落结构的动态变化及其差异性,结果表明:运行方式和染料废水浓度对敞开式白腐真菌生物反应器处理染料废水过程中生物膜微生物群落结构及其变化趋势有一定影响。序批式反应器运行的起始阶段其生物膜上黄孢原毛平革菌在数量上占绝对优势,但约1个月后逐渐侵染了热带假丝酵母、白地霉和哈茨木霉等杂菌;连续运行的白腐真菌生物反应器虽然在起始阶段存在哈茨木霉等杂菌的侵染,但约3个月后杂菌被逐渐淘汰,黄孢原毛平革菌在数量上重新占据优势,使其有利于发挥高效的降解功能。

Microbial Community Analysis of White Rot Fungal Biofilm Reac-tors for Dye Wastewater Treatment with Denaturing Gradient Gel Electrophoresis Technique

Variations of diversity of microbial genes on biofilm during reactive dye wastewater treatment were investigated using modern molecular biotechnology of the denaturing gradient gel electrophoresis(DGGE).Results showed that the microbial communities' structures of white rot fungal biofilm reactors were influenced by the operating modes(continuous and/or sequential) of the reactors and dye concentrations.Phanerochaete chrysosporium is dominant in the starting periods of sequential reactor,Candida tropicalis,Geotrichum candidum and Hypocrea lixii appeare after 1 month operation.In continuous reactor,Hypocrea lixii also appeared in the starting periods and then decreased sharply after three month's operation.Phanerochaete chrysosporium become the dominant fungi for dye degradation again.These results are very useful for white rot fungal biofilm reactor construction,starting and operational regulation.