两种苯甲酸处理系统细菌群落结构的ARDRA分析

利用ARDRA(扩增rDNA限制性分析)的方法对两种驯化条件产生的苯甲酸活性污泥中细菌的群落结构进行了分析。提取污泥样品总DNA以后,利用细菌16SrDNA通用引物进行PCR扩增。结果显示:两种活性污泥分别有20.4%和24.3%的酶切类型是自身特有的,可见在微生物群落结构上差别显著。并且利用这种方法分别检测到了103和107种的微生物类型,而利用传统的纯培养手段则只分别分离到了7种和8种微生物,分别只占ARDRA方法的6.8%和7.5%,充分显示了分子生物学的方法在进行微生物群落结构研究方面所具有的优越性。

Study on Structure of Bacteria with Two Kinds of Activated Sludge Degraded Benzoate Wastewater by ARDRA

The diversity of microorganisms associated with two kinds of activated sludge cultivated in different conditions was examined by both cultivation- based and molecular- based analysis. After total community genomic DNA was harvested from the sludge, oligonucleotides complementary to conserved regions in 16S rDNA of bacteria were used for PCR amplification. The 16S rDNA PCR products were subcloned and further characterized by a restriction fragment length analysis termed ARDRA (amplified rDNA restriction analysis). Results revealed great difference between two kinds of sludge: about 20.4% of fingerprinting is special in the library I and 24.3% in the library II. 103 and 107 kinds of fingerprinting are obtained by ARDRA, but only 7 and 8 kinds of microbials are cultured in the plate, account for 6.8% and 7.5% respectively, which revealed that ARDRA is advantageous over the diversity of microorganisms in the sludge.