| 二苯甲酮对斑点叉尾鮰鱼卵巢细胞生长的影响 |
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| 引用本文： | 王宇飞,邬红娟,文琛,刘光虹,王敏.二苯甲酮对斑点叉尾鮰鱼卵巢细胞生长的影响[J].环境科学与技术,2009,32(5). |
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| 作者姓名： | 王宇飞 邬红娟 文琛 刘光虹 王敏 |
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| 作者单位： | 1. 华中科技大学环境科学与工程学院,湖北,武汉,430074
2. 华中农业大学水产学院,湖北,武汉,430070 |
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| 基金项目： | 农业部引进国际先进农业科学技术计划(948计划)，湖北省科技攻关课题 |
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| 摘 要： | 试验通过直接、间接两种染毒手段来探讨二苯甲酮(benzophenone)对斑点叉尾鮰鱼卵巢细胞(CCO)生长的影响,以MTT法为手段测定染毒前后CCO细胞的存活数量和各阶段的生长状态,结果发现:当二苯甲酮以1～10μg/mL的浓度直接作用于CCO细胞时,CCO细胞生长在不同阶段均受到抑制作用:在指数增长期细胞生长速率减小,稳定期的存活量约为正常条件下的72%～91%,并且随着二苯甲酮浓度的增大,对CCO生长的抑制效应也相应的加剧;但是1μg/mL浓度的二苯甲酮在经过斑点叉尾鮰鱼原代肝细胞代谢后间接作用时,其代谢产物对CCO的生长在各阶段又呈现明显的促进作用,稳定期细胞数量约为正常条件下的109%。
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| 关 键 词： | 二苯甲酮 斑点叉尾鮰鱼卵巢细胞 生长影响 |
Optimum Conditions of Improved MTT Assay for Estimating in Vitro Acute Cytotoxicity in Different Fish Cells |
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| WANG Yu-fei,WU Hong-juan,WEN Chen,LIU Guang-hong,WANG Min.Optimum Conditions of Improved MTT Assay for Estimating in Vitro Acute Cytotoxicity in Different Fish Cells[J].Environmental Science and Technology,2009,32(5). |
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| Authors: | WANG Yu-fei WU Hong-juan WEN Chen LIU Guang-hong WANG Min |
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| Institution: | 1.School of Environmental Science and Engineering;Huazhong University of Science and Technology;Wuhan 430074;China;2.School of Aquatic Product;Huazhong University of Agriculture;Wuhan 430070;China |
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| Abstract: | Tests were done to determine the optimum conditions of improved MTT assay for estimating in vitro acute cytotoxicity of fish cell through Channel Catfish Ovary(CCO) cell,Ctenpharyngoden Idellus Kindy(CIK) cell and Epithelioma Papulosum Cyprinid(EPC) cell.Results showed that under the optimum conditions of wavelength 570nm and incubation time 4h,MTT concentration was 1mg/mL,with inoculation cell number depended on characteristics of cell and increased with growth speed of the cell. |
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| Keywords: | improved MTT assay fish cell in vitro acute cytotoxicity |
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