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克隆文库方法分析厌氧氨氧化反应器中细菌群落结构
引用本文:李滨,赵志瑞,马斌,张树军,刘新春,王晓辉,白志辉.克隆文库方法分析厌氧氨氧化反应器中细菌群落结构[J].环境科学与技术,2012(12):159-164,179.
作者姓名:李滨  赵志瑞  马斌  张树军  刘新春  王晓辉  白志辉
作者单位:中国科学院生态环境研究中心;河北科技大学环境科学与工程学院;中国科学院研究生院;北京市排水集团科技研发中心
基金项目:中国科学院知识创新工程重要方向性项目(KSCX2-YW-G-054-2,KZCX2-YW-JC407-3)
摘    要:为了认识低基质浓度污水厌氧氨氧化(ANAMMOX)脱氮过程的生物学机制,为ANAMMOX脱氮工艺的优化提供理论依据,通过构建细菌16S rDNA(约1 500 bp)克隆文库和浮霉菌特有16S rDNA(约830 bp)克隆文库对ANAMMOX脱氮反应器中活性污泥的细菌群落结构进行分析。从细菌16S rDNA克隆文库中随机挑选到160个克隆子,共31个分类单元(OTU),与GenBank数据库比对结果表明,厌氧颗粒污泥中的细菌群落具有丰富的多样性,包括:变形菌门(Proteobacteria)、拟杆菌门(Bacteroidete)、硝化螺旋菌门(Nitrospira)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)、Candidate division OP10、浮霉菌门(Planctomycetes)和未知菌,其中,变形菌门和拟杆菌门为优势菌群,分别占41.9%和34.2%。在浮霉菌特有16S rDNA克隆文库的40个克隆子中,34个克隆子属于CandidatusKuenenia属的厌氧氨氧化细菌,它们是ANAMMOX脱氮过程的主要功能菌。

关 键 词:厌氧氨氧化  活性污泥  16S  rDNA克隆文库  细菌群落

Analysis on Bacterial Diversity of an Anaerobic Ammonium-oxidizing Reactor by Use of 16S rDNA Clone Library
LI Bin,ZHAO Zhi-rui,MA Bin,ZHANG Shu-jun,LIU Xin-chun,WANG Xiao-hui,BAI Zhi-hui.Analysis on Bacterial Diversity of an Anaerobic Ammonium-oxidizing Reactor by Use of 16S rDNA Clone Library[J].Environmental Science and Technology,2012(12):159-164,179.
Authors:LI Bin  ZHAO Zhi-rui  MA Bin  ZHANG Shu-jun  LIU Xin-chun  WANG Xiao-hui  BAI Zhi-hui
Institution:1(1.Dept.of Environmental Biotechnology,Research Centre for Eco-environmental Sciences,CAS,Beijing 100085,China;2.College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;3.R&D Centre of Beijing Drainage Group Co.Ltd,Beijing 100124,China; 4.Graduate School of CAS,Beijing 100049,China)
Abstract:Anaerobic ammonium-oxidizing(ANAMMOX)de-nitrification reactor,featuring an activated sludge process of low substrate concentration,has been known as a cost/effective approach to de-nitrification of wastewater.To investigate its biological mechanism,a bacterial 16S rDNA clone(14 500 bp)library and a Planctomycetes-specific bacterial 16S rDNA(830 bp)library were constructed in laboratory focusing on the total and the functional bacterial diversity of activated sludge.The results indicated that bacterial diversity of the anaerobic granular sludge was very high with the communities including Proteobacteria,Bacteroidete,Nitrospira,Acidobacteria,Chloroflexi,Candidate division OP10 and Planctomycetes,inter alia,Proteobacteria and Bacteroidete were the dominant ones.Furthermore,34 clones of the 40 clones from the Planctomycetes-specific 16S rDNA library were proved to be anaerobic ammonium-oxidizing bacteria that belong to Candidatus Kuenenia,the main functional bacteria for ANAMMOX de-nitrification.
Keywords:anaerobic ammonium-oxidizing(ANAMMOX)  activated sludge  16S rDNA library  bacterial community
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