首页 | 本学科首页   官方微博 | 高级检索  
     检索      

猪肝微粒体体外代谢2,2’,4,4’-四溴联苯醚
引用本文:吴若函,沈梦楠,张圣虎,高士祥.猪肝微粒体体外代谢2,2’,4,4’-四溴联苯醚[J].环境科学与技术,2012(11):50-55.
作者姓名:吴若函  沈梦楠  张圣虎  高士祥
作者单位:污染控制与资源化研究国家重点实验室南京大学环境学院
基金项目:国家自然科学基金项目(20977044);国家“973”项目(2009CB421604)
摘    要:多溴联苯醚(PBDEs)是一类在环境中普遍存在的持久性有机污染物,研究PBDEs的体外代谢行为对理解其在体内的富集和转化具有重要意义。文章以猪肝微粒体作为研究对象,以体外代谢形式研究其对2,2’,4,4’-四溴联苯醚(BDE-47)的代谢能力,优化了代谢条件,并研究了0.01、0.05、0.1、0.5、1、5、10 mg/L的BDE-47对细胞色素P450酶系中7-乙氧基香豆素-O-脱乙基酶(ECOD)、7-乙氧基异吩唑酮-O-脱乙基酶(EROD)和苯胺4-羟基化酶(ANH)活性的影响。结果表明,孵育0.1 mg/L的BDE-47时,猪肝微粒体对BDE-47的代谢率可达到28.6%。实验所设各浓度BDE-47均能够保护微粒体的ECOD活性,但较高浓度的BDE-47(0.5、1、5 mg/L)对微粒体的EROD活性有显著抑制作用,而各浓度BDE-47对ANH活性没有显著影响。

关 键 词:微粒体  体外代谢  2  2’  4  4’-四溴联苯醚  细胞色素P450酶系

In Vitro Metabolism of 2,2’,4,4’-Tetrabromo Diphenyl Ether by Pig Liver Microsomes
WU Ruo-han,SHEN Meng-nan,ZHANG Sheng-hu,GAO Shi-xiang.In Vitro Metabolism of 2,2’,4,4’-Tetrabromo Diphenyl Ether by Pig Liver Microsomes[J].Environmental Science and Technology,2012(11):50-55.
Authors:WU Ruo-han  SHEN Meng-nan  ZHANG Sheng-hu  GAO Shi-xiang
Institution:*(State Key Laboratory of Pollution Control and Resource Reuse,School of Environment,Nanjing University,Nanjing 210046,China)
Abstract:Polybrominated diphenyl ethers(PBDEs)are a kind of persistent organic pollutants,which are ubiquitous in environment.It is of great significance to investigate metabolic behavior of PBDEs in vitro for a better understanding of their enrichment and transformation in vivo.Metabolic ability of pig liver microsomes to 2,2',4,4'-tetrabromo diphenyl ether(BDE-47)in vitro was studied.Optimal metabolic conditions and influences of BDE-47 at 0.01,0.05,0.1,0.5,1,5 and 10 mg/L on activities of 7-ethoxycoumarin O-deethylase(ECOD),7-ethoxyresorufin O-deethylase(EROD) and aniline p-hydroxylase(ANH) of cytochrome P450 enzymes were investigated.Results showed that BDE-47 could be transformed by pig liver microsomes when the exposure concentration was 0.1 mg/L,and metabolic rate was about 28.6%.BDE-47 at various concentrations set could protect the ECOD activity of microsomes,while BDE-47 at the concentration of 0.5,1 and 5 mg/L could inhibit the EROD activity significantly,and no significant effects were observed on ANH activity.
Keywords:microsomes  in vitro metabolism  2  2'  4  4'-tetrabromo diphenyl ether  cytochrome P450 enzymes
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号