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| 检测重金属离子的酶膜生物传感器的构建 | |
| 引用本文： | 寇冬梅,张进忠,杨兵,魏世强,张明晓.检测重金属离子的酶膜生物传感器的构建[J].环境科学与技术,2008,31(9). |
| 作者姓名： | 寇冬梅 张进忠 杨兵 魏世强 张明晓 |
| 作者单位： | 1. 西南大学资源环境学院,重庆,400716 2. 西南大学资源环境学院,重庆,400716;重庆市农业资源与环境重点实验室,重庆,400716 3. 重庆化工职工大学,重庆,400020 4. 西南大学化学化工学院,重庆,400716 |
| 摘 要： | 基于重金属对脲酶的特异性抑制作用,研制了可测定重金属离子的电位型酶膜生物传感器。论文考察了脲酶浓度、戊二醛浓度、温度和固定化时间等因素对电位响应的影响,获得了最佳的酶固定化条件,即酶浓度为0.8mg/mL,戊二醛为2.5%,温度为28℃,固定化时间为2h时酶固定化效果最好。同时还初步研究了该生物传感器的响应性能和再生能力。 |
| 关 键 词： | 重金属 生物传感器 脲酶 固定化 再生 |
Development of Enzymatic Membrane Biosensor for Heavy Metals Determination |
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| KOU Dong-mei,ZHANG Jin-zhong,YANG Bing,WEI Shi-qiang,ZHANG Ming-xiao.Development of Enzymatic Membrane Biosensor for Heavy Metals Determination[J].Environmental Science and Technology,2008,31(9). | |
| Authors: | KOU Dong-mei ZHANG Jin-zhong YANG Bing WEI Shi-qiang ZHANG Ming-xiao |
| Abstract: | A kind of potentiometric enzymatic membrane biosensor for determining heavy metals is developed in the lab on the basis of principle of specific heavy metals inhibition on urease. Some influencing factors were investigated such as urese and glutarladehyde concentrations, temperature and immobilization time for the optimum immobilization condition. Moreover, the response characteristics and regeneration ability of the biosensor has been preliminarily studied. |
| Keywords: | heavy metals biosensor urease immobilization regeneration |
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