| 利用ATP生物发光法快速检测丽娃河中细菌总数 |
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| 引用本文： | 张秋卓,毛映丹,杨洁,徐亚同,朱文杰.利用ATP生物发光法快速检测丽娃河中细菌总数[J].环境科学与技术,2011,34(7):152-155. |
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| 作者姓名： | 张秋卓 毛映丹 杨洁 徐亚同 朱文杰 |
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| 作者单位： | 华东师范大学上海市城市化生态过程与生态恢复重点实验室,上海,200062 |
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| 基金项目： | 上海市重大科技专项(08dz1900407); 上海市科委重大项目(09DZ1200901) |
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| 摘 要： | 利用ATP生物发光法对丽娃河中细菌总数进行了快速检测。结果表明,丽娃河中细菌总数维持在0.72×106～1.2×107CFU/mL,较综合整治前的水质有了明显地改善。研究了游离ATP以及浮游生物等对发光强度的影响,发现对样品采用过滤的方法进行预处理是提高ATP生物发光法精确度的一种重要手段。
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| 关 键 词： | 生物发光 ATP 细菌总数 快速检测 预处理 |
Fast Examination of Total Bacteria Count in Liwa Creek by ATP Bioluminescence Method |
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| ZHANG Qiu-zhuo,MAO Ying-dan,YANG Jie,XU Ya-tong,ZHU Wen-jie.Fast Examination of Total Bacteria Count in Liwa Creek by ATP Bioluminescence Method[J].Environmental Science and Technology,2011,34(7):152-155. |
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| Authors: | ZHANG Qiu-zhuo MAO Ying-dan YANG Jie XU Ya-tong ZHU Wen-jie |
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| Institution: | ZHANG Qiu-zhuo,MAO Ying-dan,YANG Jie,XU Ya-tong,ZHU Wen-jie(Key Lab of Shanghai Urbanization and Ecological Restoration,East China Normal University,Shanghai 200062,China) |
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| Abstract: | To monitor the water quality of the Liwa Creek,once a heavily polluted small river in Shanghai,grab water samples were analyzed with respect to bacteria count using a quick examination method-ATP bioluminescence.The examination process involves the studies on interferences due to free ATP and plankton in the stream water and the pretreatment by filtration of water sample,which proved indispensable for increasing the measurement accuracy. |
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| Keywords: | bio-luminescence ATP total bacteria count fast examination pretreatment |
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