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| 产氢菌Clostridium butyricum P22的分离鉴定及其产氢特性研究 | |
| 引用本文： | 潘春梅,张晓静,樊耀亭.产氢菌Clostridium butyricum P22的分离鉴定及其产氢特性研究[J].环境科学与技术,2011,34(8):9-13,17. |
| 作者姓名： | 潘春梅 张晓静 樊耀亭 |
| 作者单位： | 1. 郑州牧业工程高等专科学校生物工程系,河南郑州450011;郑州大学化学系,河南郑州450052 2. 郑州牧业工程高等专科学校生物工程系,河南郑州,450011 3. 郑州大学化学系,河南郑州,450052 |
| 基金项目： | 国家重点基础研究发展规划(973)项目(2009CB220000); 河南省教育厅自然科学研究计划项目(2010A180023); 郑州牧专博士基金项目 |
| 摘 要： | 从连续生物制氢反应器中分离得到高效产氢细菌P22,利用微生物自动鉴定仪并结合菌株16S rDNA序列分析,将其鉴定为Clostridium butyricum P22.同时还进一步研究了碳源、氮源、初始pH值和培养温度对菌株P22产氢活性的影响.结果表明:该菌株能利用多种碳源氮源产氢,当以葡萄糖作为产氢底物时,其最大产... |
| 关 键 词： | 生物制氢 Clostridium butyricum 分离鉴定 产氢特性 |
Isolation and Characterization of Hydrogen-producing Strain Clostridium butyricum P22 |
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| PAN Chun-mei,ZHANG Xiao-jing,FAN Yao-ting.Isolation and Characterization of Hydrogen-producing Strain Clostridium butyricum P22[J].Environmental Science and Technology,2011,34(8):9-13,17. | |
| Authors: | PAN Chun-mei ZHANG Xiao-jing FAN Yao-ting |
| Institution: | PAN Chun-mei1,2,ZHANG Xiao-jing1,FAN Yao-ting2(1.Department of Biotechnology,Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China,2.Department of Chemistry,Zhengzhou University,Zhengzhou 450052,China) |
| Abstract: | A hydrogen-producing strain newly isolated from anaerobic sludge in a continuously stirred anaerobic bioreactor was identified as Clostridium butyricum P22 by 16S rDNA gene sequence analysis and detection by BioMerieux Vitek.Effects of carbon sources,nitrogen source,initial pH and culture temperature on the hydrogen production with Clostridium butyricum P22 were also studied.Results showed that the strain could utilize various carbon and nitrogen source to produce hydrogen.The maximal hydrogen yield and hyd... |
| Keywords: | biohydrogen production Clostridium butyricum isolation and identification hydrogen producing characteristics |
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