| 一株石油烃降解菌的分离、鉴定及降解特性 |
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| 引用本文： | 张子间,刘勇弟,张立辉.一株石油烃降解菌的分离、鉴定及降解特性[J].环境科学与技术,2010,33(3). |
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| 作者姓名： | 张子间 刘勇弟 张立辉 |
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| 作者单位： | 1. 华东理工大学资源与环境工程学院,上海,200237;山东理工大学资源与环境工程学院,山东,淄博,255049
2. 华东理工大学资源与环境工程学院,上海,200237 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 从胜利油田采油厂石油污染土壤中分离筛选到一株石油高效降解菌SY-02,以石油烃为唯一碳源和能源。在总石油烃(TPH)浓度为1%(W/V)培养液中接种一定量的该菌,30℃、200r/min摇瓶振荡培养7d,TPH降解率可达71%。经形态、生理生化及16S rDNA同源性等指标分析,初步鉴定该菌种属Pseudomonas chlororaphis(绿针假单胞菌)。纯烃底物降解实验表明正十六烷最易被SY-02菌代谢,其次是单环芳烃苯,然后是二环芳烃萘和三环芳烃菲,环烷烃较难被利用。
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| 关 键 词： | SY-02菌株 分离鉴定 16S rDNA |
Isolation,Identification and Degradation Characteristics of Petroleum-degrading Bacteria |
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| ZHANG Zi-jian,LIU Yong-di,ZHANG Li-hui.Isolation,Identification and Degradation Characteristics of Petroleum-degrading Bacteria[J].Environmental Science and Technology,2010,33(3). |
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| Authors: | ZHANG Zi-jian LIU Yong-di ZHANG Li-hui |
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| Institution: | ZHANG Zi-jian1,2,LIU Yong-di1,ZHANG Li-hui1 (1.School of Resource and Environmental Engineering,East China University of Science and Technology,Shanghai 200237,China,2.School of Resource and Environmental Engineering,Shandong University of Technology,Zibo 255049,China) |
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| Abstract: | A strain that can grow with petroleum hydrocarbon as sole source of carbon and energy, which is SY-02, was isolated from petroleum-contaminated softs in Shengli Oil Field. Petroleum-hydrocarbon degradation was 75% after the strain was growing on inorganic salt medium with 1% (W/V) of diesel at 30℃ for 7d. The strain SY-02 which was identified as Pseudomonas chlororaphis could grow on n-bexadecane, cyclohexane, benzene, naphthalene and phenanthrene as sole source of carbon. Results also showed n-hexadecane and aromatic hydrocarbon could be degraded markedly while cyclohexane degraded not obviously. |
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| Keywords: | 16S rDNA strain SY-02 isolation and identification 16S rDNA |
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