| 同时提取土壤微生物DNA和RNA方法的研究 |
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| 引用本文： | 洪桂云,鲍立宁,魏兆军.同时提取土壤微生物DNA和RNA方法的研究[J].环境科学与技术,2011,34(6):24-27. |
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| 作者姓名： | 洪桂云 鲍立宁 魏兆军 |
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| 作者单位： | 1. 安徽建筑工业学院环境工程系,安徽合肥,230022
2. 合肥工业大学生物与食品学院,安徽合肥,230039 |
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| 基金项目： | 安徽省教育厅自然科学基金项目(KJ2008B044) |
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| 摘 要： | 获得高浓度、大片段、多样性程度高的土壤微生物总DNA是研究土壤微生物群落结构的分子生态学基础.研究采用PBS缓冲液洗涤土壤样品,结合SDS裂解微生物细胞的方法,同时提取分别添加小麦秸秆粉和油菜秸秆粉的两种土壤样晶的微生物DNA和RNA.对提取出的DNA和RNA进行酶切,基因组PCR和反转录PCR,实验结果表明该法提取的...
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| 关 键 词： | PBS洗涤土壤 同时提取DNA和RNA 酶切 16S rDNA PCR 16S rRNA反转录PCR |
Simultaneous Recovery of DNA and RNA from Soil Microbe |
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| HONG Gui-yun,BAO Li-ning,WEI Zhao-jun.Simultaneous Recovery of DNA and RNA from Soil Microbe[J].Environmental Science and Technology,2011,34(6):24-27. |
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| Authors: | HONG Gui-yun BAO Li-ning WEI Zhao-jun |
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| Institution: | HONG Gui-yun1,BAO Li-ning1,WEI Zhao-jun2(1.Department of Environmental Engineering,Anhui University of Architecture,Hefei 230022,China,2.School of Biotechnology,Hefei University of Technology,Hefei 230039,China) |
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| Abstract: | Isolation of DNA from high purity,large fragment and diversity of soil microbe is the basis for molecular ecology study on community structure.PBS washing procedure and SDS extraction method were chosen to extract DNA and RNA from two types of soils simultaneously.Enzyme digesting,PCR and RT-PCR for the extracted DNA or RNA,and results showed that purity of nucleic acid was suitable for follow-up procedures,which would provide basis for studying of microbial activities and function in soil environment. |
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| Keywords: | PBS washing procedure simultaneous recovery of DNA and RNA enzyme digesting 16S rDNA PCR 16S rRNA RT-PCR |
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