| 炼油厂周边PAHs污染土壤中微生物群落结构多样性研究 |
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| 引用本文： | 彭静静,张又弛,侯艳伟,黎慧娟,蔡超.炼油厂周边PAHs污染土壤中微生物群落结构多样性研究[J].生态环境,2011,20(5):962-965. |
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| 作者姓名： | 彭静静 张又弛 侯艳伟 黎慧娟 蔡超 |
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| 作者单位： | 1. 中国科学院城巾环境研究所//中国科学院城市环境与健康重点实验室,福建厦门,361021
2. 华侨大学化工学院环境科学与工程系,福建厦门,361021 |
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| 基金项目： | 国家自然科学基金项目(40801118) |
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| 摘 要： | 直接从污染土壤中提取微生物基因组DNA,对基因组DNA进行16S rDNA聚合酶链式反应（PCR）扩增和末端限制性片段多态性（T-RFLP）分析,进而对炼油厂附近PAHs污染区土壤微生物群落结构和多样性进行初步研究。结果表明,PAHs浓度较高的土壤中PAHs主要以高相对分子质量PAHs为主,此外,其土壤微生物群落多样性明显低于PAHs浓度较低的土壤。高浓度PAHs刺激了某些土壤微生物生长。不同污染程度的土壤存在一定数量相同的优势菌群,但相对丰度具有明显差异。其中α-变形菌是五个区域土壤中的主导微生物。研究结果将为炼油厂周边土壤的修复治理提供科学依据。
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| 关 键 词： | 土壤 PCR-T-RFLP 微生物多样性 多环芳烃 |
Application of T-RFLP to study microbial diversity in PAHs polluted soil around oil refinery |
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| PENG Jingjing,ZHANG Youchi,HOU Yanwei,Li HUIJUAN,CAI Chao.Application of T-RFLP to study microbial diversity in PAHs polluted soil around oil refinery[J].Ecology and Environmnet,2011,20(5):962-965. |
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| Authors: | PENG Jingjing ZHANG Youchi HOU Yanwei Li HUIJUAN CAI Chao |
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| Institution: | PENG Jingjing1,ZHANG Youchi1,HOU Yanwei2,Li HUIJUAN1,CAI Chao1 1.Key Lab of Urban Environment and Health//Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021,China,2.Department of Environmental Science and Technology,College of Chemical Engineering,Huaqiao University,Xiamen,361021 |
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| Abstract: | After DNA extraction from oil refinery adjacent soil directly,composition of bacterial communities was evaluated using polymerase chain reaction(PCR)-terminal restriction fragment length polymorphism(T-RFLP) analysis.Bacterial 16S rRNA T-RFLP fingerprinting revealed a high structural bacterial diversity in soil.High molecular weight PAHs is the dominate group in the high PAHs polluted soil.High concentration of PAHs in the polluted soil showed a strong effect on the bacterial populations.Our results reveale... |
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| Keywords: | soil PCR-T-RFLP microbial diversity PAHs |
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