溶藻菌发酵液及其溶藻产物的生物急性毒性试验

应用发光细菌（Photobacteriumphosphoreum）急性毒性试验，研究了溶藻菌（Streptomycessp．HJC-D1）发酵液及其对铜绿微囊藻（Microcystisaemgmosa）抑制产物的生物急性毒性。结果表明，溶藻菌发酵液本身对发光细菌具有一定的低毒性，发酵3-5d时其相对发光度为（67．59％1：3．11％）-（72．35％±2．76％）；体积分数5％的溶藻菌发酵液可有效抑制初始质量浓度高达（O．1483±0．0032）mg-L-1的铜绿微囊藻生长，其抑藻率达85％以上，且藻液毒性明显低于对照组；以微囊藻毒素为主要溶藻产物进行毒性试验发现，其半抑制质量浓度为1096．92μg·L-1，水体藻毒素质量浓度低于20μg·L-1时其生物毒性较低。

Acute toxicity of the fermentation broth of algae-lying bacteria and its products

The acute toxicity of fermentation broth of algae-lying bacteria Streptomyces sp. HJC-D1 and its products treating Microcystis aeruginosa was studied with Photobacterium phosphoreum. The results indicated that the fermentation broth of algae-lying bacteria had certain biotoxicity via photobacteria tests, and the relative luminosity was at （67.59%±3.11%）-（72.35%±2.76%） after 3-5 days＇ fermentation. The inhibitory efficiency of M. aeruginosa was above 85% at the initial concentration of （0.1483±0.0032） mg.Ll by adding fermentation broth （5%, V/V）, and it was obviously that the acute toxicity of the test groups was lower than the control groups. The toxicity tests with Microcystin-LR as the main algae-lying product showed that, the EC50 value （half inhibition concentration for 15 min） of Microcystin-LR is 1096.92 μg.L1 and there＇s no inhibited effect when the concentration of Microcystin-LR is lower than 20 μg.L-l.