苯并（a）芘对雄性罗非鱼肝脏Vtg mRNA表达的影响

卵黄蛋白原（Vitellogenin，Vtg）是检测环境雌激素的重要生物标志物，雄性鱼类VtgmRNA是检测环境雌激素的新兴生物标志物。为研究BaP是否具有环境雌激素效应，本试验对罗非鱼Oreochromis niloticus Vtg mRNA进行研究，用总RNA提取试剂盒提取得到雌鱼肝脏总RNA，以转录得到的cDNA为模板，以Primer设计得到的引物，得到422bp的罗非鱼卵黄蛋白原特异性eDNA扩增片段；采用荧光定量PCR法检测10、50Hg-L。的BaP暴露对罗非鱼肝脏Vtg基因表达的影响，结果发现4d、17d时雄性罗非鱼肝脏内VtgmRNA表达量与对照组无差异（P〉0．05），10d时10、50ug·L-1 2个剂量组VtgmRNA表达量显著高于对照组（P〈0．01），分别是对照组的113．18倍和121．79倍。结果意味着BaP可能具有环境雌激素效应。

Vtg mRNA expression in liver of male tilapia （ Oreochromis niloticus） induced by BaP

Vitellogenin （Vtg） is an important biomarker for monitoring environmental estrogens （EEs） in aquatic environment. Vtg mRNA in male fish is a new kind of biomarker. In order to research whether BaP has environmental estrogen effect, the Vtg mRNA was also studied in this paper. Total liver RNA was extracted from the liver by Trizol kit. The RT-PCR was conducted with vitellogenin special primers designed by Esterhuyse and by ourselves. The isolated 422 bp tilapia vitellogenin eDNA were obtained. The effect of BaP on tilapia liver Vtg mRNA expression was detected with the help of real-time PCR. Results showed that there were no significant differences ofVtg gene expression among control group and the exposure concentrations of 10 ~tg.L1 and 50 Bg.L-1 in day 4 and 17（P〉0.05）, in day 10, the Vtg mRNA level increased by 113.18% and 121.79% respectively in 10 ~tg-Ll group and 50 ~tg＇L-1 group which were significantly higher than that in control group （P〈0.01）.