| 水-硅油双相体系对多环芳烃降解菌的筛选及鉴定 |
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| 引用本文： | 刘芳,梁金松,李季.水-硅油双相体系对多环芳烃降解菌的筛选及鉴定[J].生态环境,2010,19(8):1887-1892. |
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| 作者姓名： | 刘芳 梁金松 李季 |
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| 作者单位： | 中国农业大学资源与环境学院,北京,100193 |
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| 基金项目： | 广东省教育部产学研结合项目 |
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| 摘 要： | 选用水-硅油双相体系驯化筛选降解多环芳烃的优势菌株。筛选体系为：50mL无机盐溶液＋10mL硅油。从74瓶富集液中,对降解效果明显的富集液进行多环芳烃降解率的液相色谱定量测定。对蒽的降解率最高为45%;对荧蒽的降解率最高为99%,几乎全部降解,对苯并a]芘的降解率为27%。筛选到一株能够高效降解蒽、菲、芘、荧蒽的菌株,编号LD29,鉴定结果为矢野口鞘氨醇杆菌LD29（Sphingobium yanoikuyae LD29）。富集液Y12对5种多环芳烃的降解效果同样很明显。
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| 关 键 词： | 水-硅油 多环芳烃 降解 筛选 |
The screening and characterization of PAHs-degrading strains by water-silicone oil system |
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| LIU Fang,LIANG Jinsong,LI Ji.The screening and characterization of PAHs-degrading strains by water-silicone oil system[J].Ecology and Environmnet,2010,19(8):1887-1892. |
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| Authors: | LIU Fang LIANG Jinsong LI Ji |
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| Institution: | ＊ College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China |
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| Abstract: | Screening of PAHs-degrading strain by using water-silicone oil biphasic system：50 mL MSM solution ＋ 10 mL silicone oil.The biodegradation of Anthracene is 45%,fluoranthene is 99%,benzoa]pyrene is 27%,by enrichment broth.We got a high biodegradation strain Sphingobium yanoikuyae LD29,an enrichment broth has the high biodegradation capacity also. |
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| Keywords: | water-silicone oil PAHs degradation screening |
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