五氯酚胁迫对转基因杨树光合光响应特性的影响

以从美国引入的转基因杨树(Populus deltoides×nigra,DN34)为材料,设置0、50、100 mg·kg~(-1)三个五氯酚胁迫质量分数开展温室盆栽试验,利用Li-6400便携式光合作用测量系统研究五氯酚污染胁迫下转基因杨树对光强的生理响应.结果表明:与对照相比,相同光强下50和100 mg·kg~(-1)质量分数的五氯酚胁迫均降低了转基因杨树叶片的净光合速率(P_n)、蒸腾速率(T_r)、胞间CO_2摩尔分数(C_i)和气孔导度(G_s).因此高质量分数五氯酚比低质量分数五氯酚对转基因杨树光合作用产生更大的抑制响应.

Photosynthesis light response characteristics of transgenic poplars under pentachlorophenol stress

To investigate photosynthetic properties of transgenic pop\ar(Populus delloides × nigra, DN34) under pentachlorophe-nol(PCP) stress. The transgenic poplars were introduced from USA. With soil culture, the transgenic poplars were cultured in the greenhouse of Chinese Academy of Forestry with different PCP stress under treatments of 0,50 and 100 mg·kg~(-1). In two months, photosynthetic parameters of second mature leaf of every transgenic poplar were determined in the same photosynthetic active radia-tion(PAR) through Li-6400 portable photosynthetic system. The results showed that compared with the control, the values of net photosynthetic rate(P_n), intercellular CO_2 concentration(C_i), transpiration rate(G_r) and stomatal conductance(G_s) decreased under the 50 and 100mg·kg~(-1) PCP stress treatment. These results implied that the high PCP concentration stress has stronger inhibitory capability of the photosynthesis for the transgenic poplar than the low PCP stress.