| 土壤中耐砷细菌的筛选和砷还原基因多样性分析 |
| |
| 引用本文： | 陈倩,苏建强,叶军.土壤中耐砷细菌的筛选和砷还原基因多样性分析[J].生态环境,2011,20(12):1919-1926. |
| |
| 作者姓名： | 陈倩 苏建强 叶军 |
| |
| 作者单位： | 中国科学院城市环境研究所,中国科学院城市环境与健康重点实验室,福建厦门361021 |
| |
| 基金项目： | 中国科学院知识创新工程青年人才类重要方向项目(KZCX2-EW-QN410); 中国科技部国际科技合作项目(2009DFB90120); 国家自然科学基金面上项目(31070101) |
| |
| 摘 要： | 采用琼脂平板培养法从湖南富砷土壤中筛选出43株耐砷细菌。16SrRNA序列分析结果表明所筛选菌分属于四个门：Actinobacteria、Proteobacteria、Firmicutes、Bacteroidetes，其中71．1％为革兰氏阳性菌。通过PCR和克隆测序等方法检测耐砷菌的砷还原相关基因（arrA、arsC、arsB／ACR3）及其基因多样性。检测结果显示：43株菌中，6．9％含异化砷还原基因arrA，30．2％含细胞质砷还原基因arsC，27．9％含As（Ⅲ）运载蛋白基因arsB／ACR3，这些基因在细菌中的出现频率较低。通过Mega软件构建系统发育树发现arrA基因的多样性可能受一定的地域差异影响，arsC基因在一些菌株中存在着基因水平转移现象，同时表明a变形菌可能更倾向于拥有Acr3型As（Ⅲ）载体蛋白，而arsB则多出现在芽孢杆菌中。
|
| 关 键 词： | 耐砷菌 异化砷还原基因arrA 细胞质砷还原基因arsC As（Ⅲ）运载蛋白基因arsB／ACR3 基因多样性 |
Isolation of arsenic-resistant bacterias from soil and the diversity of arsenate reducing genes |
| |
| Chen Qian
,
Su Jianqiang
,
Ye Jun.Isolation of arsenic-resistant bacterias from soil and the diversity of arsenate reducing genes[J].Ecology and Environmnet,2011,20(12):1919-1926. |
| |
| Authors: | Chen Qian Su Jianqiang Ye Jun |
| |
| Institution: | Chen Qian,Su Jianqiang,Ye Jun Key Lab of Urban Environment and Health,Institute of Urban Environment,Chinese Academy of Sciences,Xiamen 361021 |
| |
| Abstract: | Forty-three arsenic-resistant bacteria were isolated by agar plates from arsenic-contaminated soil from Hunan Province.The 16S rRNA sequence analysis results showed that these strains belonged to four phyla : Actinobacteria,Proteobacteria,Firmicutes and Bacteroidetes,and 71.1 percent of them were gram-positive bacteria.PCR amplification and sequencing methods were used for detecting the occurrence of some functional genes related to arsenate reduction(arrA,arsC,arsB/ACR3) and the gene diversity.The results ... |
| |
| Keywords: | Arsenic-resitant bacteria Arsenate respiratory reductase gene arrA Cytoplasmic arsenate reductase gene arsC Arsenite transporter genes arsB/ACR3 Gene diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
