碳基纳米铜复合材料对普通小球藻胁迫作用的研究

随着科技的飞速发展,纳米类材料在电化学、药物运输、生物传感器等领域中得到了广泛的应用,这导致了其通过各种途径进入水、土壤、大气等环境介质中,对生态环境安全造成潜在威胁。以水体中环境浓度的碳基纳米铜复合材料为受试浓度范围,以环境适应性和污染物耐受性均较强的普通小球藻(Chlorella vulgaris)为对象,研究了水体中不同浓度碳基纳米铜复合材料对普通小球藻的细胞胁迫作用,使用分光光度法检测了0,0.05,0.5,5,50 mg·L^-1碳基纳米铜暴露条件下普通小球藻叶绿素a含量的变化。运用非靶向代谢组学(气相-质谱联用)检测法探究了不同浓度碳基纳米铜胁迫条件下第8天普通小球藻细胞内代谢物相对丰度变化、代谢通路及相关代谢组学特性的变化。结果表明,在培养周期的第8天,藻细胞叶绿素a含量随碳基纳米铜浓度的升高而降低,当碳基纳米铜达到50 mg·L^-1时,将严重抑制藻细胞叶绿素a的产生。代谢组学检测结果表明,当碳基纳米铜复合材料的浓度达到50 mg·L^-1时,藻细胞中与叶绿素合成密切相关的碳固定过程、氨基酸代谢过程均受到了严重抑制。这表明,高浓度碳基纳米铜会扰乱普通小球藻的细胞代谢并产生明显胁迫作用。文章旨在评估水体中碳基纳米铜复合材料对水体初级生产者普通小球藻的胁迫作用,以期为水体污染修复与藻细胞生态毒理学的研究提供一定的数据支持与参考。

Stressed effects of C-Cu2O nanoparticles on Chlorella vulgaris

With the rapid development of science and technology,nano materials have been widely used in various fields such as electrochemistry,drug transport and biosensors.The nanoparticles inevitably enter water,soil,and atmosphere,potentially threatening the security of the ecosystem.In this study,the stressed effects of C-Cu₂O nanoparticles on Chlorella vulgaris(C.vulgaris)was investigated.The concentration of chlorophyll-a(Chl-a)was determined using 721 spectrophotometry under the C-Cu₂O nanoparticlesexposed environmental concentration range from 0.05 mg·L^-1to 50 mg·L^-1(0,0.05,0.5,5,50 mg·L^-1).In addition,changes of relative abundance of intracellular metabolites,metabolic pathways and related metabonomic characteristics of(C.vulgaris)under different concentrations of C-Cu₂O nanoparticles exposure on the 8 th day were investigated by Label-Free metabonomic.The results demonstrated that the chl-a concentration decreased with the increase of C-Cu₂O nanoparticles concentration.The concentrations of chl-a were severely inhibited when it reached 50 mg·L^-1.Furthermore,the results of metabolomic indicated that the processes of carbon fixation in photosynthesis and amino acid metabolism which were related to chl-a synthesis were severely inhibited.The above results demonstrated that high concentration of C-Cu₂O nanoparticles will disturb the normal metabolism of C.vulgaris and threaten the growth of the algal cell.The present study aims to evaluate the stressed effect of C-Cu₂O nanoparticles in aquatic environment on C.vulgaris which is one of the primary producers,then provide data support and reference for the exploration of water pollution restoration and algal ecotoxicology.