| 转野生荠菜凝集素基因棉花对赤子爱胜蚓的影响 |
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| 引用本文： | 丁帅,方志翔,刘标,沈文静,孟军.转野生荠菜凝集素基因棉花对赤子爱胜蚓的影响[J].生态与农村环境学报,2012,28(4):389-393. |
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| 作者姓名： | 丁帅 方志翔 刘标 沈文静 孟军 |
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| 作者单位： | 1. 环境保护部南京环境科学研究所国家环境保护生物安全重点实验室,江苏南京210042;南京农业大学生命科学学院,江苏南京210095
2. 环境保护部南京环境科学研究所国家环境保护生物安全重点实验室,江苏南京,210042 |
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| 基金项目： | 转基因生物新品种培育科技重大专项 |
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| 摘 要： | 在实验室条件下分别给赤子爱胜蚓(Eisenia foetida)饲喂在自然状况下可能接受的棉叶最高添加量和2倍最高添加量的转野生荠菜凝集素(WSA)基因棉花(Gossypium hirsutum)叶片,并以其亲本棉花叶片为对照,以牛粪为阴性对照,以农药为阳性对照,研究种植转WSA基因棉花对赤子爱胜蚓死亡率、生长及繁殖的影响。结果表明,在49 d的试验周期内,与取食亲本棉花叶片的赤子爱胜蚓相比,取食转WSA基因棉花叶片的赤子爱胜蚓的死亡率,体质量,GST、SOD、纤维素酶活性以及蚓茧数和小蚯蚓数均无显著差异,未发现转WSA基因棉花对赤子爱胜蚓有不利影响。
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| 关 键 词： | 转野生荠菜凝集素基因棉花 赤子爱胜蚓 谷胱甘肽硫转移酶 超氧化物歧化酶 纤维素酶 繁殖 |
Effects of Wild-Shepherd's-Purse-Agglutinin ( WSA ) Transgenic Cotton on Eisenia foetida |
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| DING Shuai
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FANG Zhi-xiang
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LIU Biao
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SHEN Wen-jing
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MENG Jun.Effects of Wild-Shepherd's-Purse-Agglutinin ( WSA ) Transgenic Cotton on Eisenia foetida[J].Journal of Ecology and Rural Environment,2012,28(4):389-393. |
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| Authors: | DING Shuai FANG Zhi-xiang LIU Biao SHEN Wen-jing MENG Jun |
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| Institution: | 1(1.Nanjing Institute of Environmental Sciences,Ministry of Environmental Protection,Nanjing 210042,China;2.College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Groups of earth worms(Eisenia foetida) were raised in laboratory.Group Ⅰ was fed with the highest naturally acceptable dose of wild shepherd’s purse agglutinin(WSA) transgenic cotton(Gossypium hirsutum) leaves,Group Ⅱ with twice as much,Group Ⅲ with non-transgenic parent cotton leaves as control,Group Ⅳ with cow dung as negative control and Group Ⅴ with pesticide as positive control.The objective of the study is to explore effects of planting WSA transgenic cotton on survival,growth,and reproduction of E.foetida.Results of the 49 d experiment show that there was no significant difference in death rate,weight,SOD activity,GST activity,cellulase activity,number of earthworm cocoons and number of baby earthworms between Group Ⅲ and Groups Ⅰ and Ⅱ,which suggests that WSA transgenic cotton does not have any significant adverse effects on E.foetida. |
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| Keywords: | wild shepherd’s purse agglutinin(WSA) transgenic cotton Eisenia foetida SOD GST cellulase reproduction |
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