Effects of lead exposure on redox status,DNA and histological structures in Venus verrucosa gills and digestive gland |
| |
Authors: | Safa Bejaoui Khaoula Telahigue Imene Chetoui Wafa Trabelsi Imen Rabeh Salwa Nechi |
| |
Institution: | 1. Ecology, Biology and Physiology laboratory of Aquatic Organisms, Biology Department, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisiasafa.bejaoui@fst.utm.tnhttps://orcid.org/0000-0002-7946-2763;3. Ecology, Biology and Physiology laboratory of Aquatic Organisms, Biology Department, Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia;4. Anatomy and Cytology Service, Mohamed Taher Maamouri hospital, Nabeul, Tunisia |
| |
Abstract: | ABSTRACTLead (Pb) is one of the most toxic heavy metals that affect the physiological status of aquatic organisms. The present investigation evaluated the possible toxic effect of lead chloride (PbCl2) on biomarkers responses, DNA damage and histological alterations in Venus verrucosa gills and digestive gland. Three concentrations of PbCl2 (D1:1µgL?1, D2: 10µgL?1 and D3: 100µgL?1) were chosen for V. verrucosa exposure during six days. At the end of the trial, it was found that Pb tended to accumulate in both gills and digestive gland in a dose-dependent manner. However, gill tissues exhibited the highest metal burden. Our results showed an increase of malondialdehyde, protein carbonyls and advanced oxidation protein product levels in both organs following PbCl2exposure. The induction of both non-enzymatic and enzymatic antioxidant systems; as well as the decrease of the acetylcholinesterase activity and degradation of DNA structure was recorded in the gills and digestive gland. The histopathological alterations observed in gills (disruption of lamellas and cilia filaments?…) and digestive gland (lumens occlusion, necrosis and fibrosis) confirmed the aforementioned results. Our data highlighted the short-term toxicity effects of PbCl2 on V. verrucosa and pointed out a high sensitivity of gills towards this metal. |
| |
Keywords: | Antioxidants defenses histological injuries lead exposure macromolecules damages Venus verrucosa |
|
|