| 用简并引物扩增与克隆D.radiodurans超氧化物歧化酶和过氧化氢酶基因片段 |
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| 引用本文： | 吕星,邢瑞云,郑晖,姚启智.用简并引物扩增与克隆D.radiodurans超氧化物歧化酶和过氧化氢酶基因片段[J].应用与环境生物学报,1999,5(5):673. |
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| 作者姓名： | 吕星 邢瑞云 郑晖 姚启智 |
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| 作者单位： | 1. 北京放射医学研究所,北京,100850
2. 中国科技大学研究生院,北京,100039 |
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| 摘 要： | 依据超氧化物歧化酶（ＳＯＤ） 和过氧化氢酶（Ｃａｔ） 的保守性氨基酸序列，设计简并引物，自Ｄ．ｒａｄｉｏｄｕｒａｎｓ 基因组ＤＮＡ扩增并克隆了ＳＯＤ和Ｃａｔ 基因片段．ＳＯＤ 和Ｃａｔ 基因片段分别长４５３ ｂｐ 和６７３ ｂｐ，各编码１５１和２２４ 个氨基酸，氨基酸序列与不同生物来源的ＳＯＤ或Ｃａｔ 具有高度同源性
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| 关 键 词： | einococcusradiodurans 超氧化物歧化酶 过氧化氢酶 |
| 修稿时间： | 1999-03-09 |
AMPLIFICATION AND CLONING OF DEINOCOCCUS RADIODURANS SUPEROXIDE DISMUTASE AND CATALASE GENE FRAGMENTS WITH DEGENERATE OLIGONUCLEOIDE PRIMERS |
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| L Xing,XING Ruiyun,ZHENG Hui,YAO Qizhi.AMPLIFICATION AND CLONING OF DEINOCOCCUS RADIODURANS SUPEROXIDE DISMUTASE AND CATALASE GENE FRAGMENTS WITH DEGENERATE OLIGONUCLEOIDE PRIMERS[J].Chinese Journal of Applied and Environmental Biology,1999,5(5):673. |
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| Authors: | L Xing XING Ruiyun ZHENG Hui YAO Qizhi |
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| Institution: | L(U) Xing,XING Ruiyun,ZHENG Hui,YAO Qizhi |
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| Abstract: | Both superoxide dismutase (SOD) and catalase (Cat) gene fragments from the extraordinary radioresistant bacterium Deinococcus radiodurans were cloned and their sequences were determined. The fragments were generated by PCR analysis of D. radiodurans genomic DNA with degenerate oligonucleoide primers based on the conservative regions of the known SODs or Cats. The 453 bp length SOD and 672 bp length Cat gene fragments consist of 151 and 224 amino acids, respectively, which share considerable homology with other SODs or Cats. |
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| Keywords: | Deinococcus radiodurans superoxide dismutase catalase |
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