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| 黄河三角洲湿地土壤微生物群落结构分析 | |
| 引用本文： | 刘芳,叶思源,汤岳琴,滝川清,木田建次,吴晓磊.黄河三角洲湿地土壤微生物群落结构分析[J].应用与环境生物学报,2007,13(5):691-696. |
| 作者姓名： | 刘芳 叶思源 汤岳琴 滝川清 木田建次 吴晓磊 |
| 作者单位： | 1. 清华大学环境科学与工程系,北京,100084 2. 青岛海洋地质研究所,山东青岛,266071 3. 熊本大学工学院,日本熊本,860-0862 |
| 摘 要： | 采用末端限制性片段长度多态性分析(T-RFLP)技术和16S rDNA克隆文库的方法,分析了黄河三角洲滨海湿地土壤不同深度细菌和古菌的群落结构.研究表明,随着深度的增加,细菌群落的多样性下降,而古菌群落多样性则有上升的趋势,且土壤的细菌和古菌群落结构都呈现出规律的层状分布.该土壤包括各种硫酸盐还原菌、产甲烷古菌、光合细菌等丰富的细菌和古菌资源.图5参27 |
| 关 键 词： | 滨海湿地 微生物群落结构 末端限制性片段长度多态性分析 16SrDNA克隆文库 种群多样性 |
| 收稿时间： | 2007-04-20 |
| 修稿时间： | 2007-05-22 |
Analysis of Microbial Community Structure in Coastal Wetland Soil of the Yellow River Delta |
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| LIU Fang,YE Siyuan,TANG Yueqin,TAKIGAWA Kiyoshi,KIDA Kenji,WU Xiaolei.Analysis of Microbial Community Structure in Coastal Wetland Soil of the Yellow River Delta[J].Chinese Journal of Applied and Environmental Biology,2007,13(5):691-696. | |
| Authors: | LIU Fang YE Siyuan TANG Yueqin TAKIGAWA Kiyoshi KIDA Kenji WU Xiaolei |
| Institution: | I Department of Environmental Science and Engineering, Tsinghua Unviersity, Beijing 100084, China;21nstitute of Marine Geology, Qingdao 266071, Shandong, China;3 Faculty of Engineering, Kumamoto University, Kumamoto 860-0862, Japan |
| Abstract: | Terminal restriction fragment length polymorphism and 16S ribosomal DNA(rDNA)clone library analyses were conducted to assess bacterial and archaeal diversities and community structures at different soil layers of the coastal wetland in the Yellow River Delta region.Shannon-Wiener index showed that the species richness of bacteria decreased with depth,while the richness of archaea tended to increase.Classification analysis showed that the bacterial and archaeal community structures subjected to stratified distribution.The wetland soil was very rich in resources of bacteria and archaea,including SRB,methanogenic archaea and phototrophic bacteria.Fig 5,Ref 27 |
| Keywords: | coastal wetland microbial community structure terminal restriction fragment length polymorphism(T-RFLP) 16S rDNA clone library species diversity |
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