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| 螺孢菌ZG9901的筛选及其产碱性果胶酶发酵条件研究 | |
| 引用本文： | 钟卫鸿,王启军,岑沛霖.螺孢菌ZG9901的筛选及其产碱性果胶酶发酵条件研究[J].应用与环境生物学报,2000,6(5):468-472. |
| 作者姓名： | 钟卫鸿 王启军 岑沛霖 |
| 作者单位： | 1. 浙江工业大学生物与环境工程学院杭州 310014 2. 浙江大学食品生物技术学院杭州 310021 3. 浙江大学生物工程研究所杭州 310027 |
| 基金项目： | 浙江省自然科学基金部分资助项目 |
| 摘 要： | 用筛选培养基从芦苇土壤中筛选到７株碱性果胶酶产生菌株，经初筛选和复筛得到产酶活性较高的一株菌株ＺＧ９９０１，初步鉴定为螺孢菌属Ｓｐｉｒｉｌｌｏｓｐｏｒａ ｓｐｐ．最适摇瓶产酶条件是：果胶２０ｇ／Ｌ，乳糖２０ｇ／Ｌ，蛋白胨３ｇ／Ｌ，酵母膏４ｇ／Ｌ，ＫＨ２ＰＯ４１ｇ／Ｌ，ＭｇＳＯ４．７Ｈ２Ｏ０．０４ｇ／Ｌ，ＭｇＣｌ２０．２ｇ／Ｌ，ｐＨ９．０，３２℃培养３６ｈ达到产酶高峰。 |
| 关 键 词： | 碱性果胶酶 螺孢菌 发酵条件 |
| 修稿时间： | 2000年4月24日 |
STUDIES ON ALKLINE PECTINASE PRODUCING CONDITIONS BY SPIRILLOSPORA |
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| Zhong Weihong,Wang Qijun,Cen Peilin.STUDIES ON ALKLINE PECTINASE PRODUCING CONDITIONS BY SPIRILLOSPORA[J].Chinese Journal of Applied and Environmental Biology,2000,6(5):468-472. | |
| Authors: | Zhong Weihong Wang Qijun Cen Peilin |
| Abstract: | 7 alkaline pecti nase producing strains were isolated from soil. Strain ZG9901 with high alka line pectinase activity was obtained by repeated screening. It was found that ZG 9901 belongs to Spirillospora and its optimum enzymeproducing medium is: 20 g/L pectin, 20 g/L lactose, 3 g/L peptone, 4 g/L yeast extrac t, 3 g /L KH2PO4, 0.2 g/L MgCl2, 0.04 g/L MgSO47H2O, pH 9.0. After 36 h hours ' shake culture at 32℃, the maximum yield of enzyme was obtained. Fig 7, Tab 5, Ref 7 |
| Keywords: | alkaline pectinase Spirillospora spp fermentation condition |
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