转bar及NIa基因白菜的田间生物学及经济性状调查

对转入除草剂抗性基因bar及来自病毒TuMV的／VIa基因的不结球白菜纯合株系二代进行了田问生物学性状调查,结果表明，转基因白菜的植物学性状与其未转基因对照材料相比，无明显差别；但在涉及其生存竞争力的一些生物学性状，如发芽势、花粉活力、种子库存力等方面，比对照材料弱，其经济性状也比对照稍差．获得的转基因性状——除草剂抗性，能稳定遗传，并在田间很好表达；NIa基因介导的TuMV抗性在露地开放栽培条件下，不能很好表达，转基因株染病较重．对产生以上现象的原因进行了分析讨论．图3表5参16

EVALUATION OF TRANSGENIC CHINESE CABBAGE UNDER FIELD CONDITIONS

Four homozygous transgenic Chinese cabbage (Brassica rapa ssp. chinensis var. utilis Tsen et Lee) lines were evaluated in the field. The plants carried a bar gene for Basta resistance and a NIa gene from TuMV for virus resistance. A number of biological and agronomic characteristics, including growth duration, pollen vigor, seeds per pod, seed germination capacity, yield of flower stem and seed storage ability, were evaluated under field conditions. The result showed that most of the traits were statistically comparable between the transformed and nontransformed plants except some traits, such as seed vigor, pollen vigor and seed preserve ability. They were related to the competition talent and declined in transgenic plants.The yield of flower stem was also lower than the control. The transgene mediated Basta resistance of the transgenic plants expressed well in the field conditions, but the virus resistance did not. One hybridized combination with good traits was bred between a transgenic line and a traditional variety. The inbreeding depression of the transgenic plants and the complex pathological environment in the field were suggested to be the main reason for the phenomena above. Fig 3, Tab 5, Ref 16