| 发光细菌法检测环境污染中的基因毒性 |
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| 引用本文： | 赵华清,殷浩文,陈晓倩,郭锐,顾宗濂,谢思琴.发光细菌法检测环境污染中的基因毒性[J].应用与环境生物学报,2000,6(6):577-580. |
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| 作者姓名： | 赵华清 殷浩文 陈晓倩 郭锐 顾宗濂 谢思琴 |
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| 作者单位： | 1. 上海市环境科学研究院生态毒理实验室上海 200233
2. 中国科学院南京土壤研究所南京 210008 |
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| 基金项目： | 上海市环境保护科学基金资助项目（97-006） |
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| 摘 要： | 用明亮发光杆菌Ｔ３小种的暗变种Ｔ９１７１菌株检测９种化合物和１４种环境样品的基因毒性。结果表明，在９种化合物中检出３种阳性物，６种可疑物。在１４种环境样品中检出２个样品呈阳性，７个样品呈可疑，其余为阴性。激活系统Ｓ９能极大地提高试验的灵敏度。在环境基因毒物的筛选中，发光细菌法是一种非常有价值的短期生物试验方法。表２参１４
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| 关 键 词： | 基因毒性 发光细菌法 检测 环境污染 |
| 修稿时间： | 2000年3月8日 |
SCREENING GENOTOXICOTY ON ENVIRONMENTAL SAMPLE BY PHOTOBACTERIA DARK MUTANT |
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| ZHAO Huaqing,YIN Haowen,Chen Xiaoqian,GUO Rui,GU Zonglian,XIE Siqin.SCREENING GENOTOXICOTY ON ENVIRONMENTAL SAMPLE BY PHOTOBACTERIA DARK MUTANT[J].Chinese Journal of Applied and Environmental Biology,2000,6(6):577-580. |
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| Authors: | ZHAO Huaqing YIN Haowen Chen Xiaoqian GUO Rui GU Zonglian XIE Siqin |
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| Abstract: | In this study, the genotoxicity on 9 chemicals and 14 environmental samples was detected by strain T9171, photobacterium phosphoreum T3 dark mutant. The result showed 3 chemicals and 2 environmental samples were judged positive substance, 6 chemicals and 7 environmental samples were suspicion substance, 5 environmental samples were negative substance. The sensitivity of the method was improved by S9 addition. In short term bioassays, it is a very valualle technique for screening genotoxicity. Tab 1, Ref 14 |
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| Keywords: | S9 genotoxicity photobacteria dark mutant test S9 |
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