| 呋喃丹降解菌CFDS-1的筛选鉴定及降解特性 |
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| 引用本文： | 徐剑宏,洪青,洪源范,李顺鹏.呋喃丹降解菌CFDS-1的筛选鉴定及降解特性[J].应用与环境生物学报,2011(2):237-242. |
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| 作者姓名： | 徐剑宏 洪青 洪源范 李顺鹏 |
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| 作者单位： | 江苏省农业科学院食品质量安全与检测研究所农业部食品质量安全监控重点开放实验室;南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室; |
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| 基金项目： | 国家自然科学基金项目(Nos.30901006,30400013)资助~~ |
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| 摘 要： | 为有效治理呋喃丹的污染,从受呋喃丹长期污染的土壤中分离筛选到一株高效降解呋喃丹的菌株CFDS-1,经形态、生理生化、16S rDNA(GenBank accession No.AY702969)同源性及系统发育地位等分析,将其初步鉴定为Sphingomonas sp.当接种量为5%时,CFDS-1能在48 h内降解100 mg L-1的呋喃丹,对于高达300 mg L-1的呋喃丹依然有降解效果;CFDS-1对呋喃丹的降解率与起始接种量呈正相关;降解呋喃丹的最适pH是8.0～9.0;在20～42℃范围内,温度对CFDS-1降解呋喃丹没有显著影响;该菌在250 mL三角瓶中装液量为100 mL时,对呋喃丹的降解效果最好.土壤实验表明,该菌株同样能有效地降解土壤中的呋喃丹残留.
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| 关 键 词： | Sphingomonas sp. 呋喃丹 生物降解 生物修复 |
Isolation,Identification and Degradation of Carbofuran-degrading Strain CFDS-1 |
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| XU Jianhong,HONG Qing,HONG Yuanfang & LI Shunpeng.Isolation,Identification and Degradation of Carbofuran-degrading Strain CFDS-1[J].Chinese Journal of Applied and Environmental Biology,2011(2):237-242. |
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| Authors: | XU Jianhong HONG Qing HONG Yuanfang & LI Shunpeng |
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| Institution: | XU Jianhong1,HONG Qing2,HONG Yuanfang2 & LI Shunpeng2(1Key Laboratory of Food Safety Monitoring and Management,Ministry of Agriculture,Institute of Food Safety,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)(2Key Laboratory of Microbiological Engineering of Agricultural Environment,College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Carbofuran(2,3-dihydro-2,2-dimethyl-7-benzofuranoyl N-methylcarbamate) was first introduced into China in 1979 as a pesticide and is extensively used as a soil-incorporated N-methylcarbamate insecticide to control a variety of insect pests that infest many kinds of crops,such as corn and potato.Carbofuran is of a concern due to its relatively high mammalian toxicity and relatively high mobility in soil.Some N-methylcarbamate compounds and their metabolites can be sufficiently mobile in certain soil,thus con... |
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| Keywords: | Sphingomonas sp carbofuran biodegradation bioremediation |
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