| ‘香玲’核桃试管苗生长条件的优化 |
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| 引用本文： | 冀爱青,朱超,吴国良.‘香玲’核桃试管苗生长条件的优化[J].应用与环境生物学报,2011(3):359-363. |
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| 作者姓名： | 冀爱青 朱超 吴国良 |
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| 作者单位： | 山西农业大学园艺学院;晋中学院生物科学与技术学院;河南农业大学园艺学院; |
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| 基金项目： | 河南省科技厅重大科技攻关项目(No.092101110600)资助~~ |
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| 摘 要： | 为优化核桃试管苗生长条件,向核桃优良品种规模化栽培提供大量优良的种植材料,以‘香玲’核桃实生苗为材料,对基本培养基的类型(MS,DKW,WPM)和凝固剂种类(琼脂,Phytagel,二者结合)进行了筛选,并采用完全随机试验设计研究了细胞分裂素种类和浓度对其试管苗生长的影响.试验结果表明:基本培养基与凝固剂的种类,以及细胞分裂素的种类与浓度对芽苗生长的影响很大.以DKW为基本培养基,2.2 g/L Phytagel为凝固剂,激素配比为0.8 mg/L BA+0.01 mg/L IBA,有利于芽苗增殖,增殖可达4.70;后在0.8 mg/L KT+0.01 mg/L IBA进行壮苗,即可进行生根培养.不同基因型对基本培养基中无机盐成分的需求不同,含有较丰富矿物质的Phytagel有利于芽苗生长.BA促进腋芽的萌动和增殖,而KT则有利于壮苗.图4表3参22
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| 关 键 词： | ‘香玲’核桃 试管苗 基本培养基 凝固剂 细胞分裂素 增殖 伸长 |
Optimization of Growth Conditions of in Vitro Shoots of Walnut 'Xiangling' |
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| JI Aiqing,ZHU Chao & WU Guoliang.Optimization of Growth Conditions of in Vitro Shoots of Walnut 'Xiangling'[J].Chinese Journal of Applied and Environmental Biology,2011(3):359-363. |
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| Authors: | JI Aiqing ZHU Chao & WU Guoliang |
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| Institution: | JI Aiqing1,2,ZHU Chao3 & WU Guoliang3(1Faculty of Horticulture,Shanxi Agricultural University,Taigu 030801,Shanxi,China)(2Faculty of Biology Science and Technology,Jinzhong University,Jinzhong 030600,China)(3Faculty of Horticulture,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | To provide abundant and excellent colonial plant materials,the optimum growth conditions of in vitro walnut shoot were studied.Using the seedlings of walnut 'Xiangling' as materials,the types of nutrient media(MS,DKW and WPM) and gelling agents(Difco Bacto agar,Phytagel and a mixture of Phytagel and Difco Bacto agar) were screened,and the effects of different types and concentrations of cytokinin on growth of the seedlings were studied with randomized complete-block design.The results indicated that differe... |
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| Keywords: | walnut 'Xiangling' in vitro shoot basic medium gelling agent cytokinin proliferation elongation |
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