| 水稻DDB1基因的表达特性及功能分析 |
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| 引用本文： | 罗晓莉,牛向丽,黄维藻,刘永胜.水稻DDB1基因的表达特性及功能分析[J].应用与环境生物学报,2011(1):1-5. |
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| 作者姓名： | 罗晓莉 牛向丽 黄维藻 刘永胜 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室; |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项课题(Nos.2009ZX08009-072B,2009ZX08001-011B); 国家杰出青年科学基金项目(No.30825030)资助~~ |
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| 摘 要： | 利用RNA干涉技术构建水稻DDB1(Damaged DNA binding protein 1)基因不同启动子驱动植物表达载体,DDB1-RNAi和DDB1-glu-RNAi.通过根癌农杆菌介导转入水稻愈伤,经组织培养成功获得转基因植株.半定量RT-PCR分析显示,与野生型植株相比,两组转基因植株幼叶内DDB1的表达量...
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| 关 键 词： | DDB1基因 RNA干扰 果实特异启动子 农杆菌介导转化 雄性半不育 水稻 |
Expression Characteristics and Functional Analysis of Rice DDB1 Gene |
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| LUO Xiaoli,NIU Xiangli,HUANG Weizao & LIU Yongsheng.Expression Characteristics and Functional Analysis of Rice DDB1 Gene[J].Chinese Journal of Applied and Environmental Biology,2011(1):1-5. |
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| Authors: | LUO Xiaoli NIU Xiangli HUANG Weizao & LIU Yongsheng |
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| Institution: | LUO Xiaoli,NIU Xiangli,HUANG Weizao & LIU Yongsheng(Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China) |
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| Abstract: | In this study,two RNA interference(RNAi) vectors of rice DDB1 gene,DDB1-RNAi and DDB1-glu-RNAi,droved by different promoters were constructed.They were introduced into rice cv.Nipponbare by Agrobacterium-mediated transformation.The analysis of semi-quantitative RT-PCR revealed the variation of suppression of DDB1 in both RNAi transgenic lines compared to that of wild type.I2-KI indicated abnormally stained pollens,high level male semi-sterile in two sets of transgenic plants,and DDB1-RNAi transformed lines ... |
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| Keywords: | DDB1 RNA interference fruit-specific promoter Agrobacterium-mediated transformation male semi-sterile rice |
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