| 十字花科蔬菜BcMF13同源基因的克隆及进化分析 |
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| 引用本文： | 李焰焰,聂传朋,曹家树.十字花科蔬菜BcMF13同源基因的克隆及进化分析[J].应用与环境生物学报,2011(2):207-210. |
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| 作者姓名： | 李焰焰 聂传朋 曹家树 |
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| 作者单位： | 阜阳师范学院生命科学学院;浙江大学蔬菜研究所细胞与分子生物学实验室; |
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| 基金项目： | 国家自然科学基金项目(No.31000132); 安徽省高校青年教师项目(No.2008jq1121)资助~~ |
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| 摘 要： | 在十字花科芸薹属的8个物种中扩增到与十字花科蔬菜花粉发育相关的一个重要基因BcMF13(GenBank登陆号:EF158459)的同源序列,获得8个同源序列的DNA序列全长602～607 bp,均含有一个内含子区,编码73个氨基酸,在内含子区内存在1个插入/缺失、5处颠换、4处转换.对8个同源基因的氨基酸比对发现该基因在十字花科物种内有很强的保守性.基于Kimura双参数距离,构建了BcMF13同源基因在十字花科中进化的NJ系统树,反映出南方大白菜与北方大白菜存在不同的演化过程.
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| 关 键 词： | 十字花科 芸薹属 同源基因 进化分析 BcMF13 |
Cloning and Evolutionary Analysis of Homologous Sequences of BcMF13 Gene in Cruciferous Vegetables |
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| LI Yanyan,NIE Chuanpeng & CAO Jiashu.Cloning and Evolutionary Analysis of Homologous Sequences of BcMF13 Gene in Cruciferous Vegetables[J].Chinese Journal of Applied and Environmental Biology,2011(2):207-210. |
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| Authors: | LI Yanyan NIE Chuanpeng & CAO Jiashu |
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| Institution: | LI Yanyan1,2,NIE Chuanpeng1 & CAO Jiashu2(1School of Life Sciences,Fuyang Teachers College,Fuyang 236041,Anhui,China)(2Laboratory of Cell and Molecular Biology,Institute of Vegetable Science,Zhejiang University,Hangzhou 310029,China) |
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| Abstract: | 8 sequences homologous to BcMF13(GenBank number: EF158459) were cloned from Brassica of Cruciferae.The results showed that the 8 DNAs were 565 bp long and contained open reading frames(ORF) of 222 bp that encoded 73 amino acids.They all contained an intron with 1 insertion/deletion site,f ive transversion sites and four transition sites.The 8 deduced amino acid sequences were very conservative in Cruciferous species.NJ tree of BcMF13 homologous genes was constructed based on Kimura two-parameter distance.Th... |
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| Keywords: | Cruciferae Brassica homologous gene evolutionary analysis BcMF13 |
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