| 长柄石杉居群遗传多样性和遗传结构的AFLP分析 |
| |
| 引用本文： | 张君诚,宋育红,朱勇,张杭颖,钟扬.长柄石杉居群遗传多样性和遗传结构的AFLP分析[J].应用与环境生物学报,2011(1):18-23. |
| |
| 作者姓名： | 张君诚 宋育红 朱勇 张杭颖 钟扬 |
| |
| 作者单位： | 三明学院应用生物技术研究所;复旦大学生物多样性与生态工程教育部重点实验室; |
| |
| 基金项目： | 福建省科技厅重点科技项目(No.2010N0027); 福建省自然科学基金(No.2008J0301); 福建省高校服务海西重点项目(No.HX200801)资助~~ |
| |
| 摘 要： | 石杉科植物因所含石杉碱甲(Huperzine A)对中老年痴呆等具有良好疗效,近年来倍受关注.利用AFLP分子标记对武夷山脉广布种长柄石杉Huperzia serrata(Thunb.ex Murray)Trev.var.longipetiolata(Spring)H.M.Chang]7个居群112株个体进行遗传多样性和居群遗传结构分析.选用多态性高、分辨力强的8对选择性扩增引物组合共获得675个位点,其中多态位点比例为69.38%.居群内观测等位基因数(Na)为1.633,有效等位基因数(Ne)1.493;Nei’s基因多样性指数(He)与Shannon多态性信息指数(I)的平均值分别为0.272和0.392,多样性最高为地处武夷山脉中段的泰宁居群和建宁居群,最低为山脉北段的光泽居群.居群总基因多样性(Ht)为0.327 3,居群内基因多样性(Hs)为0.272 2,居群间的遗传分化系数(Gst)为0.168 1,表明居群内变异是长柄石杉遗传多样性的主要来源.由Gst估计,武夷山脉长柄石杉自然居群的基因流(Nm)为2.474 0.邻接树分析表明居群间遗传亲缘关系与地理位置相关.武夷山脉长柄石杉较高的遗传多样性和基因流水平表明其仍然具有相当的适应(生存)能力和进化潜力,这可能与其生物学(异交水平)、生态学特性及武夷山脉相对良好的生境条件有关.
|
| 关 键 词： | 长柄石杉 AFLP 遗传多样性 居群 遗传结构 武夷山 |
AFLP Analysis of Genetic Diversity and Population Structure of Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang |
| |
| ZHANG Juncheng,SONG Yuhong,ZHU Yong,ZHANG Hangying & ZHONG Yang.AFLP Analysis of Genetic Diversity and Population Structure of Huperzia serrata (Thunb. ex Murray) Trev. var. longipetiolata (Spring) H. M. Chang[J].Chinese Journal of Applied and Environmental Biology,2011(1):18-23. |
| |
| Authors: | ZHANG Juncheng SONG Yuhong ZHU Yong ZHANG Hangying & ZHONG Yang |
| |
| Institution: | ZHANG Juncheng1,2,SONG Yuhong1,ZHU Yong2,ZHANG Hangying1 & ZHONG Yang2(1Institute of Applied Biotechnology,Sanming College,Sanming 365004,Fujian,China)(2Key Laboratory of Biodiversity Science & Ecology Engineer,Ministery of Education,Fudan University,Shanghai 200433,China) |
| |
| Abstract: | Huperziaceae has attracted the attention of researchers in recent years because of its Huperzine A(HupA) being a potent,reversible and selective acetylcholinesterase inhibitor(AChEI).The genetic diversity and genetic structure of seven populations and 112 individuals of Huperzia serrata(Thunb.ex Murray) Trev.var.longipetiolata(Spring) H.M.Chang from the Wuyi Mountains were analyzed by amplified fragment length polymorphism(AFLP).We recorded a total of 675 amplified bands using 8 pairs of AFLP primers,555 of... |
| |
| Keywords: | Huperzia serrata(Thunb ex Murray) Trev var longipetiolata(Spring) H M Chang AFLP genetic diversity population genetics genetic structure Wuyi Mountains |
| 本文献已被 CNKI 等数据库收录！ |
|
