| 糯稻和非糯稻蜡质基因的新STS分子标记 |
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| 引用本文： | 孙华钦,田洁,郑家奎,蒋开锋,罗科.糯稻和非糯稻蜡质基因的新STS分子标记[J].应用与环境生物学报,2006,12(4):461-463. |
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| 作者姓名： | 孙华钦 田洁 郑家奎 蒋开锋 罗科 |
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| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041
2. 四川农业科学院水稻高粱研究所,四川泸州,646100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 通过对前人公布的糯性水稻蜡质基因全序列测定结果的分析,根据糯性水稻与非糯性水稻蜡质基因序列位点的差异,设计了两个基于PCR扩增反应的可特异识别糯性水稻的显性和共显性STS分子标记;同时在前人公布的非糯性水稻蜡质基因的不同类型(Wxa和Wxb)的全序列比对分析的基础上,选取了两种基因型特定的差异位点,设计了两个基于PCR扩增反应用于特异识别这两种Wx基因的显性STS分子标记,并用新建立的标记对相应的水稻基因型进行了检测.图2表2参10
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| 关 键 词： | 直链淀粉含量(AC) 糯性水稻 非糯性水稻 STS分子标记 蜡质基因 |
| 收稿时间： | 2005-03-22 |
| 修稿时间： | 2005-06-10 |
New STS Molecular Markers for Waxy Gene of Glutinous and Nonglutinous Rices |
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| SUN Huaqin,TIAN Jie,ZHENG Jiakui,JIANG Kaifeng,LUO Ke.New STS Molecular Markers for Waxy Gene of Glutinous and Nonglutinous Rices[J].Chinese Journal of Applied and Environmental Biology,2006,12(4):461-463. |
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| Authors: | SUN Huaqin TIAN Jie ZHENG Jiakui JIANG Kaifeng LUO Ke |
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| Institution: | 1 Chengdu Institute of Biology, Chinese Academy ofScienees, Chengdu 610041, China;2 Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646100, Sichuan, China |
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| Abstract: | After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene. Moreover, the whole sequences of the two Wx genes (Wx~ a and Wx~ b ) of nonglutinous rice reported by others were also aligned, and another two pairs of dominant STS molecular markers were designed to discriminate Wx~ a from Wx~ b based on the special site between the two genotypes. Then, corresponding rice genotypes were identified by the established new markers. Fig 2, Tab 2, Ref 10 |
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| Keywords: | amylose content (AC) glutinous rice nonglutinous rice STS molecular marker Wx gene |
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