白腐菌Trametes hirsute对六氯苯的降解及其条件优化

在液体体系下,筛选了5株白腐菌,对六氯苯进行了降解研究,并优化了白腐菌Trametes hirsute TH对六氯苯的降解条件.结果表明,不同白腐菌均能降解六氯苯,其中白腐菌Trametes sp.TR对六氯苯降解率最高,达90.21%;而白腐菌T.hirsute TH对六氯苯降解率为87.08%,但生物量最高,达3.33 g/L.通过响应面法优化白腐菌T.hirsute TH降解六氯苯的条件,结果显示,转速、接种量和培养时间是影响六氯苯降解的主要因素.优化后的最佳条件为:转速125 r/min,菌丝接种量8%(V/V),培养时间2 d,温度28 ℃,pH为7.在优化条件下,2 d内白腐菌T.hirsute TH对浓度为10 mg/L的六氯苯降解率和降解量分别可达91.52%和2.288 mg L-1 d-1.图2表7参17

Biodegradation of Hexachlorobenzene by Trametes hirsute and ptimization of Its Culture Conditions

Degradation characteristics of HCB by five strains of white rot fungi and optimization of degradation conditions of HCB by T. Hirsute TH in liquid culture system were investigated. The results showed that the five strains could degrade HCB effectively, the degradation rate of HCB by Trametes sp. strain TR, which could reach 90.21%, was the highest and the biomass of T. hirsute was as high as 3.33 g/L although the degradation rate was 87.08 %. Aiming to obtain the optimum degradation conditions, response surface methodology (RSM) was used to optimize the degradation conditions of HCB by T. hirsute TH. Three factors, rotational speed, inoeulum volume and culture time, mainly affected HCB biodegradation by the fungus. The maximum degradation rate of HCB by T. hirsute TH could be achieved under rotational speed of 125 r/min, inoculum volume of 8% (V/V), culture time of 2 days, temperature of 28 ℃ and pH of 7. Under these optimum conditions, the degradation rate and amount of HCB with 10 mg/L by T. hirsute TH were 91.52% and 2.288 mg L~(-1) d~(-1), respectively. Fig 2, Tab 7, Ref 17