长期继代培养马铃薯愈伤组织的植株再生

对影响马铃薯愈伤组织发生、生长和长期继代培养的愈伤组织再生植株的几种因素进行了研究。马铃薯茎段的愈伤组织发生频率高于叶片的愈伤组织发生频率。Ｄｉｃａｍｂａ诱导茎段和叶片的愈伤组织发生频率在９０％以上；而２，４－Ｄ的诱导效果不佳并诱发外植体生根。再生培养其中加入０．５ｇ／Ｌ的酶解酷蛋白是必需的；蔗糖的浓度以２％－４％为宜；附加ＺＴ２．０ｍｇ／Ｌ、ＧＡ０．１ｍｇ／Ｌ可使长期继代的愈伤组织的植株再生频率

PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO

The factors effecting callus initiation and growth, as well as plant regeneration from long-term cultured calli of potato(Solanum tuberosum L.) were studied. The frequency of callus initiation from stem segments was higher than that from leaf discs. The frequency of callus initiation by Dicamba was about 90% from stem segments and leaf discs, but that by 2, 4-D was lower, and roots were induced on the explants. It was necessary to supply casein hydrolasate at 0.5 g/L in the regeneration medium. The suitable concentration of sucrose was 2% -4% . The regeneration frequency of long-term cultured calli could reach 80% when ZT 2.0 mg/L and GA0.1mg/L were supplied in the regeneration medium. Of the three tested genotypes.only one could reproduce plants. Furtheremore ,secondary calli were also induced and could reproduce plants.