| 黄地老虎NPV增效作用的研究 |
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| 引用本文： | 白小东,丁翠.黄地老虎NPV增效作用的研究[J].应用与环境生物学报,2000,6(1):52-55. |
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| 作者姓名： | 白小东 丁翠 |
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| 作者单位： | 中国科学院动物研究所,北京,100080 |
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| 基金项目： | 国家九·五攻关项目，国家开放实验室课题资助 |
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| 摘 要： | 对黄地老虎核型多角体病毒（ＡｓＮＰＶ）的多角体蛋白和病毒粒子组分进行分离纯化，分别与棉铃虫核型多角体病毒（ＨａＮＰＶ）混合感染三龄棉铃虫幼虫，发现此二种组分均能够提高棉铃虫幼虫的死亡率，缩短半致死时间，降低半致死浓度。使用酶联免疫吸附测定（ＥＬＩＳＡ）技术检验ＡｓＮＰＶ多角体蛋白和病毒粒子与粘虫颗粒体病毒增效因子（ＰｓＧＶ－ＳＦ）和ＨａＮＰＶ多角体蛋白的同源性，发现ＡｓＮＰＶ多角体蛋白和病毒粒子与
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| 关 键 词： | 增效机制 黄地老虎NPV 棉铃虫NPV 生物农药 |
STUDY ON SYNERGISTIC ACTION OF AGROTIS SEGETUM NUCLEAR POLYHEDROSIS VIRUS |
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| BAI Xiaodong,DING Cui.STUDY ON SYNERGISTIC ACTION OF AGROTIS SEGETUM NUCLEAR POLYHEDROSIS VIRUS[J].Chinese Journal of Applied and Environmental Biology,2000,6(1):52-55. |
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| Authors: | BAI Xiaodong DING Cui |
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| Abstract: | Polyhedrin
and virion, which were isolated and purified from Agrotis setegum nuclear polyhedrosis
virus (AsNPV), are administered to 3-instar Heliothis armigera larvae with Heliothis
armigera nuclear polyhedrosis virus (HaNPV). Both polyhedrin and virion could increase
mortality of H. armigera larvae, shorten half mortality time and decrease half mortality
concentration. Enzyme-Linked Immunosorbent Assay was used to study the homologies between
polyhedrin,virion and synergistic factor of Pseudaletia separata Granulosis Virus
(PsGV-SF),polyhedrin of HaNPV. Poor homology was discovered between AsNPV
polyhedrin,virion and PsGV-SF. AsNPV polyhedrin shows high homology with HaNPV polyhedrin.
Synergistic mechanism of AsNPV is discussed. Fig 6, Tab 1, Ref 9 |
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| Keywords: | Agrotis setegum nuclear polyhedrosis virus co-infection homology synergistic mechanism |
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