| 铁皮石斛种质资源的低温离体保存 |
| |
| 引用本文： | 史永忠,潘瑞炽,王小菁,叶庆生,郭丽荣.铁皮石斛种质资源的低温离体保存[J].应用与环境生物学报,2000,6(4):326-330. |
| |
| 作者姓名： | 史永忠 潘瑞炽 王小菁 叶庆生 郭丽荣 |
| |
| 作者单位： | 华南师范大学生物系,广东省植物发育生物工程重点实验室,广州,510631 |
| |
| 基金项目： | 广东省科学技术委员会重点资助 |
| |
| 摘 要： | 研究了在低温条件下保存铁皮石斛种质资源的方法和影响因素。结果表明,铁皮石斛苗在４℃黑暗条件下可连续保存１２个月,并能恢复下正常生长。１／２ＭＳ和１／４ＭＳ培养基上的存活率高于ＭＳ。蔗糖深度以２０－４０ｇ／Ｌ为宜。添加低浓度活性炭可明显改善保存材料的状态。甘露醇对铁皮石斛试管苗的保存效果不佳。
|
| 关 键 词： | 铁皮石斛 种质资源 离休积存 |
IN VITRO CONSERVATION OF DENDROBIUM OFFICINALE AT LOW TEMPERATURE |
| |
| SHI Yongzhong,PAN Ruichi,WANG Xiaojing,YE Qingsheng,GUO Lirong.IN VITRO CONSERVATION OF DENDROBIUM OFFICINALE AT LOW TEMPERATURE[J].Chinese Journal of Applied and Environmental Biology,2000,6(4):326-330. |
| |
| Authors: | SHI Yongzhong PAN Ruichi WANG Xiaojing YE Qingsheng GUO Lirong |
| |
| Abstract: | The
method and influencing factors of in vitro conservation of Dendrobium officinale germplasm
were studied. Seedlings could be conservedcondition in vitro for 12 months at 4℃ in dark, without subculture. The
survival rates on 1/2 MS and 1/4 MS media were higher than that on MS medium. The suitable
concentrations of sucrose were 20 to 40 g/L. Supplement with activated charcoal could
improve the growth conditions of stored plantlets. However, mannitol was not beneficial to
the conservation of D.officinale. Plate 1, Fig 1, Tab 3, Ref 21 |
| |
| Keywords: | Dendrobium officinale germplasm in vitro conservation |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
