| 褐牙鲆白化相关基因zfp123的组织表达谱与蛋白结构分析 |
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| 引用本文： | 王家庆,侯林,齐遵利,李代宗,钟尉方,姚锋.褐牙鲆白化相关基因zfp123的组织表达谱与蛋白结构分析[J].应用与环境生物学报,2008,14(6). |
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| 作者姓名： | 王家庆 侯林 齐遵利 李代宗 钟尉方 姚锋 |
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| 作者单位： | 1. 河北农业大学海洋学院,秦皇岛,066003
2. 辽宁师范大学生命科学学院,大连,116029 |
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| 基金项目： | 辽宁省科技基金资助(No.2050819)项目 Supported by the Science and Technology Foundation of Liaoning |
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| 摘 要： | C2H2锌指是真核细胞中最常见的DNA结合模体.对通过mRNA差异显示技术和克隆测序.在褐牙鲆中获得的一条白化相关锌指蛋白基因zfp123,进行了电子表达谱与实验验证表达谱的比较分析及该蛋白序列的一、二级和三级结构分析.电子表达谱分析分别对与ZFP123有较高同源性的斑马鱼、家鼠和猪的zfnd5a(zfp123)摹因各个组织表达情况进行了比较分析;一、二级结构分析发现其具有多个保守的基序和结构域;同源建模显示其具有一个"C-X(2-4)-CX11-C-X2-C"结构的锌指样三级结构.ZFP可以介导靶基因的转录调控,抑制或激活特定基因的表达;对DNA进行修饰,如人造限制性内切酶、重组酶、抗病毒感染等.锌指蛋白应用前景广阔.研究价值显著,是未来基因治疗的革命性的工具.
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| 关 键 词： | 褐牙鲆 白化相关基因 组织表达谱 蛋白结构 |
Tissue Expression Profi le and Protein Structure Analysis of Albinism-related Gene of zfp123 from Paralichthys olicaceus |
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| WANG Jiaqing,HOU Lin,QI Zunli,LI Daizong,ZHONG Weifang,YAO Feng.Tissue Expression Profi le and Protein Structure Analysis of Albinism-related Gene of zfp123 from Paralichthys olicaceus[J].Chinese Journal of Applied and Environmental Biology,2008,14(6). |
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| Authors: | WANG Jiaqing HOU Lin QI Zunli LI Daizong ZHONG Weifang YAO Feng |
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| Institution: | WANG Jiaqing1,HOU Lin2,QI Zunli2,LI Daizong1,ZHONG Weifang1 & YAO Feng2 (1College of Ocean,Hebei Agriculture University,Qinhuangdao 066003,Hebei,China) (2College of Life Sciences,Liaoning Normal University,Dalian 116029,Liaoning,China) |
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| Abstract: | C2H2 zinc finger is one of the most common eukaryotic DNA binding motifs. An albinism-related gene of zfp123 of Paralichthys olicaceus was acquired by mRNA differential display, cloned and sequenced. The comparative analysis of electronic expression profile and experimental verif ication expression profile, and the analyses of the primary, secondary and tertiary structures of the protein were performed. The comparative analysis of electronic expression profi le of zfand5a gene from zebrafish, house mouse an... |
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| Keywords: | Paralichthys olicaceus albinism-related gene tissue expression profile protein structure |
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